We present a combined macro-scale/micro-scale computational approach to quantify oxygen transport and flow-mediated shear stress to human chondrocytes cultured in three-dimensional scaffolds in a perfusion bioreactor system. A macro-scale model was developed to assess the influence of the bioreactor design and to identify the proper boundary conditions for the micro-scale model. The micro-scale...

We have developed a chip-based cell culture system for the three-dimensional cultivation of cells. The chip is typically manufactured from non-biodegradable polymers, e.g., polycarbonate or polymethyl methacrylate by micro injection molding, micro hot embossing or micro thermo-forming. But, it can also be manufactured from bio-degradable polymers. Its overall dimensions are 0.7 1 x 20 x 20 x 0....

A current focus of biopharmaceutical research and production is seed train process intensification. This allows for intermediate cultivation steps to be avoided or even the direct inoculation a bioreactor with cells from cryovials cryobags. Based on preliminary investigations regarding suitability high cell densities cryopreservation perfusion cultivations as inoculum further cultivations, an u...

Bioreactors are growing in popularity among network engineers. generally chambers used for cell culture processes with predetermined parameters. Pivot is a bioreactor system that controlled automatically and remotely using multi-chambers allow inflow of fresh liquid or output sample collection. This facilitates gas exchange between the medium ambient gas. The provides storage bag media peristal...

Cancer is one of the most prevalent and disruptive diseases affecting population, as such, subject major research efforts. Recently, these efforts have been put towards understanding role that exosomes can play in progression cancer. Exosomes are small extracellular vesicles ranging from 40–150 nm size carry bioactive molecules like proteins, DNA, RNA, miRNA, surface receptors. One important fe...

BACKGROUND High proliferative and differentiation capacity renders embryonic stem cells (ESCs) a promising cell source for tissue engineering and cell-based therapies. Harnessing their potential, however, requires well-designed, efficient and reproducible expansion and differentiation protocols as well as avoiding hazardous by-products, such as teratoma formation. Traditional, standard culture ...

The perfusion mode of a continuous cell culture bioreactor was modified to establish a closed loop system. Eighty percent of the spent medium was re-used twice. The medium cycle bioreactor unit was operated sterile and uncomplicated without a technical retention system for the high molecular weight substances. Therefore, only 20% of the actual medium was necessary to run the recycling process. ...

INTRODUCTION: Surgical repair of articular cartilage typically yields fibrocartilage that lacks the stratified ECM architecture of native cartilage and does not integrate with the surrounding hyaline cartilage. Because of these limitations, there has been considerable interest in cartilage tissue engineering and that led to the development of various bioreactor systems to grow functional engine...

Nutrient transport limitation remains a fundamental issue for in vitro culture of engineered tissues. In this study, perfusion bioreactor configurations were investigated to provide uniform delivery of oxygen to media equivalents (MEs) being developed as the basis for tissue-engineered arteries. Bioreactor configurations were developed to evaluate oxygen delivery associated with complete transm...

The accurate prediction of hepatotoxicity demands validated human in vitro models that can close the gap between preclinical animal studies and clinical trials. In this study we investigated the response of primary human liver cells to toxic drug exposure in a perfused microscale 3D liver bioreactor. The cellularized bioreactors were treated with 5, 10, or 30 mM acetaminophen (APAP) used as a r...