PURPOSE For periodontal tissue engineering, it is a primary requisite and a challenge to select the optimum types of cells, properties of scaffold, and growth factor combination to reconstruct a specific tissue in its natural form and with the appropriate function. Owing to fundamental disadvantages associated with using a two-dimensional substrate, several methods of seeding cells into three-d...

Regenerative medicine and tissue engineering technologies have greatly benefitted medicine and dentistry over the past years (Zaky & Cancedda 2009). The use of protein-, geneand stem cell-based therapeutics have been exploited significantly in the reconstruction of new tissues and organs (Discher et al. 2009). The field of oral and periodontal regenerative medicine has undergone significant rec...

Tooth loss or damage, such as that caused by dental caries and periodontal disease, can cause fundamental problems with oral functions. The development of regenerative therapy for tooth tissue repair and whole-tooth replacement is currently considered a novel treatment with the potential to fully recover tooth function. Several mesenchymal stem cell-like cell types have been identified in oral ...

Cementum is a mineralized tissue produced by cementoblasts covering the roots of teeth that provides for the attachment of periodontal ligament to roots and surrounding alveolar bone. To study the mechanism of proliferation and differentiation of cementoblasts is important for understanding periodontal physiology and pathology including periodontal tissue regeneration. However, the detailed mec...

Periodontal ligament-associated protein-1 (PLAP-1)/asporin is a recently identified novel member of the small leucine-rich repeat proteoglycan family. PLAP-1/asporin is involved in chondrogenesis, and its involvement in the pathogenesis of osteoarthritis has been suggested. We report that PLAP-1/asporin is also expressed specifically and predominantly in the periodontal ligament (PDL) and that ...

background  wisdom teeth are usually removed as a routine procedure for therapeutic or prophylactic reasons. developing wisdom teeth are easy accessible source of stem cells during the adulthood which could be obtained. methods  search of the pubmed for stem cells and tooth was done. an inclusion criterion was extract of stem cells from wisdom tooth. results  stem cells can be obtained from den...

Although cells derived from periodontal ligament (PDL) tissue are reported to have stem cell-like activity and are speculated to play a crucial role for tissue healing and regeneration after injury or orthodontic treatment, mechanisms regulating their recruitment and activation remain unknown. Recently, stromal cell-derived factor 1α (SDF-1α) has been reported to be important for stem cell homi...

Avulsion injury is one of the most severe types traumatic dental injuries. Following avulsion, periodontal ligament tissues are injured and vessels nerves pulp rupture at apical foramen which causes necrosis. In studies it was reported that key to retention knocked-out teeth maintain viability ligament. Storage media plays an important role in preserving PDL cells during extra alveolar time. Th...

Enamel matrix derivative (EMD), a porcine extract harvested from developing porcine teeth, has been shown to promote formation of new cementum, periodontal ligament and alveolar bone. Despite its widespread use, an incredibly large variability among in vitro studies has been observed. The aim of the present study was to determine the influence of EMD on cells at different maturation stages of o...

Fluoride, which is often added to toothpaste or mouthwash in order to protect teeth from decay, may be a novel therapeutic approach for acceleration of periodontal regeneration. Therefore, we investigated the effects of fluoride on proliferation and mineralization in human periodontal ligament cells in vitro. The periodontal ligament cells were stimulated with various concentrations of NaF adde...