Background/Aims: The gut flora may play an important role in the pathogenesis of inflammatory bowel disease. An ileal reservoir or pouch can be created to replace the excised rectum after proctocolectomy. In patients with ulcerative colitis this is subject to inflammation and termed pouchitis. Using bacteria from patients the authors sought evidence for the presence rather than the identity of ...

Many patients with COVID-19 experience a range of debilitating symptoms months after being infected, syndrome termed long-haul COVID. A 68-year-old male presented lung opacity, fatigue, physical and cognitive weaknesses, loss smell lymphocytopenia. After rounds therapeutic plasma exchange (TPE), the patient returned to normal activities work. Mechanistically in patient’s peripheral blood...

BACKGROUND DNA repair is a cellular defence mechanism responding to DNA damage caused in large part by oxidative stress. There is a controversy with regard to the effect of red blood cells on DNA damage and cellular response. AIM To investigate the effect of red blood cells on H2O2-induced DNA damage and repair in human peripheral blood mononuclear cells. METHODS DNA breaks were induced in ...

background: atherosclerosis is a multifactorial disorder with chronic inflammatory conditions in which immune cells play a significant role in its pathogenic process. regulatory t cells (treg), as a part of immune system, are involved in controlling autoimmune and inflammatory diseases. quantitative and/or functional alteration of tregs has been shown to play an atheroprotective role and may al...

BACKGROUND/AIMS The gut flora may play an important role in the pathogenesis of inflammatory bowel disease. An ileal reservoir or pouch can be created to replace the excised rectum after proctocolectomy. In patients with ulcerative colitis this is subject to inflammation and termed pouchitis. Using bacteria from patients the authors sought evidence for the presence rather than the identity of a...

Citation: Terlizzi M, Molino A, Colarusso C, Donovan C, Imitazione P, Somma P, Aquino RP, Hansbro PM, Pinto A and Sorrentino R (2018) Activation of the Absent in Melanoma 2 Inflammasome in Peripheral Blood Mononuclear Cells From Idiopathic Pulmonary Fibrosis Patients Leads to the Release of Pro-Fibrotic Mediators. Front. Immunol. 9:670. doi: 10.3389/fimmu.2018.00670 activation of the absent in ...

References 1. Wang M, Sun H, Zhang W, Zhang Y. Functional activation of Proline-rich Tyrosine Kinase2 (PYK2) in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. BMC Musculoskelet Disord. 2009;10:141. 2. Wang M, Zhang W, Zhang Y. Activation signal transduction by Proline-rich Tyrosine Kinase 2 (PYK2) in Peripheral blood mononuclear cells from patients with syst...

Little information is available on the specific role of alveolar macrophages (AMs) in modulating local cellular reactions to inhaled allergens in atopic asthma. We investigated the influence of alveolar macrophages obtained by bronchoalveolar lavage (BAL) on the proliferative responses of lavage and peripheral lymphocytes from 12 patients with atopic asthma, 6 nonasthmatic symptomatic atopic su...

Homologous restriction factor (HRF) is a 65-kDa membrane protein that inhibits transmembrane channel formation by the membrane-attack complex of complement and by the complement component C9-related cytolytic lymphocyte protein. Stimulation of resting peripheral human lymphocytes with the anti-CD3 monoclonal antibody OKT3 has been shown to induce cytotoxicity in the CD8+ subpopulation. As demon...

To produce an in vitro model of nemaline myopathy, we reprogrammed the peripheral blood mononuclear cells (PBMCs) a patient with heterozygous p.Gly148Asp mutation exon 3 ACTA1 gene to iPSCs. Using CRISPR/Cas9 editing corrected generate isogenic control line. Both mutant and show normal karyotype, express pluripotency markers could differentiae into three cell states that represent embryonic ger...