A barley (Hordeum vulgare cv Igri) genomic library was screened with synthetic oligonucleotides that correspond to the noncoding sequences of a barley PLTP cDNA (Molina and García-Olmedo, 1993). Plaque hybridizations were performed at moderate stringency with 32P-end-labeled oligonucleotides. Nitrocellulose filters were exposed to film for 16 h at -80°C. A positive plaque was isolated, and the ...

Special AT-rich sequence binding protein 1 (SATB1) has regulatory effects on gene expression and appears to play an important role in tumor progression. The present study screened the promoter region of the SATB1 gene for polymorphisms, evaluated the corresponding haplotypes regarding alterations in promoter activity in vitro and analyzed the impact of these haplotypes on the clinical course of...

The ecdysteroid UDP-glucosyltransferase (egt) gene promoter fragments of different lengths were generated from the genomic DNA of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) by PCR. After being purified and enzymatic digestion, they were cloned into the pGEM-3Z vector for construction of reporter plasmids pAcegt542-luc, pAcegt309-luc and pAcegt159-luc with the luciferas...

Transformation vector For [SM1]4, a synthetic gene coding for four units of the SM1 peptide (PCQRAIFQSICN) separated by 4-amino-acid (GSPG) linkers was constructed as follows. Two oligonucleotides, SM1þ (5 0 -CCCGTGCCAGCGCGCCATCTTCCAGTCGATCTGCAA CGGCTCGCCGGG-3 0 ) and SM1 (5 0 -GCCCGGCGAGCCGTTGCAGATCGACTGGAA GATGGCGCGCTGGCACGG-3 0 ), were annealed, phosphorlylated and self-ligated. The ligation...

Exposure of DNA to several proteins peroxidized by radiation-generated hydroxyl free radicals resulted in formation of crosslinks between the macromolecules, detected by retardation and broadening of DNA bands in agarose gels. This technique proved suitable for the study of crosslinking of DNA with peroxidized BSA, insulin, apotransferrin and alpha casein, but not with several other proteins, i...

Name of the Sequence. Ovine lipoprotein lipase cDNA. Genus and Species. Ovis aries. Origin of Clones. Because almost the whole 3′-untranslated region (3′UTR) was found to be lacking from the ovine lipoprotein lipase (LPL) cDNA sequence (1,656 bp including the 5′UTR, the coding sequence, and the first 44 bp of the 3′UTR) published by Edwards et al. (1993), we have undertaken to characterize this...

The monoamine oxidase A (MAOA) is involved in the degradation of various biogenic amines which have been hypothesized to have a relationship with personality traits. In literatures, however, inconsistent results appeared in a number of case-control studies using the functional MAOA-uVNTR marker. We aimed to further look into the uVNTR polymorphism among the Asian population and to preliminarily...

OBJECTIVES The basic HLH transcription factor Olig is a key regulator for differentiating the oligodendrocyte lineage cells during development. Oligodendrocyte transcription factor 2 (Olig2) plays a crucial role in differentiating the oligodendrocytes in the spinal cord. We aimed to construct and investigate the eukaryotic expression recombinant plasmid in the rat Olig2. DESIGN, TIME AND SETT...

Influenza virus A (H1N1) is an important subtype of influenza virus that makes numerous consequences throughout the world. In the first step of viral attachment, main antigenic site of the HA1 domain from the globular head of hemagglutinin (HA) binds to human cell receptors, starting the disease process. In order to produce recombinant subunit protein vaccines, we focus on the nucleotide sequen...

BACKGROUND CD19 is a pan B cell marker that is recognized as an attractive target for antibody-based therapy of B-cell disorders including autoimmune disease and hematological malignancies. The object of this study was to stably express the human CD19 antigen in the murine NIH-3T3 cell line aimed to be used as an immunogen in our future study. METHODS Total RNA was extracted from Raji cells i...