Methods Three eighty seven (387) patients who presented to the HIV clinic with symptoms of lower abdominal pain, itching and abnormal vaginal discharge were selected after obtaining written informed consent. Patients on oral or vaginal medications for vaginitis were excluded. High vaginal/cervical swabs were collected, cultured and processed using standard microbiological methods. Anti-microbia...

An endoglucanase encoded by a gene of Clostridium josui was expressed in Escherichia coli and purified. The homogeneous enzyme, with a molecular weight of 39,000, revealed maximum endoglucanase activity at pH 7.2 to 7.5 and a temperature of 65 to 70 degrees C. The enzyme was stable at a temperature lower than 45 degrees C (the growth temperature of the bacterium) in the range of pH 4.5 to 9.0. ...

8 Sterk PJ, Fabbri LM, Quanjer PH, et al. Airway Responsiveness: Standardised challenge testing with pharmacological, physical and sensitizing stimuli in adults. Eur Respir J 1993; 6: Suppl. 16, 53–83. 9 Quanjer PH, Stanojevic S, Cole TJ, et al. Multi-ethnic reference values for spirometry for the 3–95 year age range: the global lung function 2012 equations. Eur Respir J 2012; 40: 1324–1343. 10...

Objective: A simple, specific, accurate and precise Rp-high-performance liquid chromatography method was developed and validated for determination of Eprosartan Mesylate in pharmaceutical preparations. Materials and Methods: The method showed a linear response for concentrations in the range of 2-12 μg/mL using Methanol: Acetonitrile: Buffer solution (Dissolve 0.02 M potassium di-hydrogen ortho...

OBJECTIVE Pulmonary hypertension (PH) has been described in hemodialysis (HD) patients and has been associated with increased morbidity and mortality. Our objective was to determine the prevalence of pulmonary hypertension in patients on regular hemodialysis. METHODS This cross sectional study was conducted in Department of Nephrology, Liaquat National Hospital Karachi from April 2013 to Marc...

Resolution of binary mixtures of metoprolol and propranolol without sample pretreatment has been successfully achieved, using three different methods. The first method was based on application of Vierodt’s method that involves measurements of the absorbances at 222.6 and 213.4 nm for metoprolol and propranolol. Calibration graphs were established in the range of 1-70 μg/mL and 0.5-30 μg/mLfor m...