Production of recombinant rat Vegf: Vegf cDNA, amplified from a rat cDNA library, was cloned into the pPICZαA secretion vector and expressed using the Pichia pastoris yeast expression system, according to the instructions of the manufacturer (Invitrogen, Carlsbad, CA). After overnight dialysis of the yeast-conditioned medium against 10 mM acetic acid (pH 5.5), Vegf was purified by sequential ch...

BACKGROUND Independent of their blood pressure lowering effect, ACE inhibitors are thought to reduce vascular inflammation. The clinical relevance of this effect is unclear with the current knowledge. Abdominal aortic aneurysms (AAA) are characterized by a broad, non-specific inflammatory response, and thus provide a clinical platform to evaluate the anti-inflammatory potential of ACE inhibitor...

the incidence of brain neoplasm has been progressively increasing in recent years in the industrialized countries. one of the reasons for this increased incidence could be better access to health care and improved diagnosis in the industrialized countries. it also appears that caucasians have a higher incidence than blacks or hispanics or asians. a number of risk factors have been identified an...

investors use different approaches to select optimal portfolio. so, optimal investment choices according to return can be interpreted in different models. the traditional approach to allocate portfolio selection called a mean - variance explains. another approach is markov chain. markov chain is a random process without memory. this means that the conditional probability distribution of the nex...

fault tree analysis (fta) as a probabilistic risk assessment (pra) method is used to identify basic causes leading to an undesired event, to represent logical relation of these basic causes in leading to the event, and finally to calculate the probability of occurrence of this event. to conduct a quantitative fta, one needs a fault tree along with failure data of the basic events (bes). someti...

risks mean cases of uncertainty of project, the impact of which is realized as a threat (negative aspect) and/or opportunity (positive aspect). the traditional viewpoint on risk is a negative viewpoint that implies damages, loss and harmful consequences. judgments such as this on risk merely emphasize on risks management and pay less attention to opportunities management. it is clear that some ...

selecting an effective project plan is a significant area in the project management. the present paper introduces a technique to identify the project plan efficient frontier for assessing the alternative project plans and selecting the best plan. the efficient frontier includes two criteria: the project cost and the project time. besides, the paper presents a scheme to incorporate directed acyc...