The spread of carbapenemase-producing gram-negative bacilli is a global public health problem. Several authors have proposed phenotypic assays to presumptively detect these enzymes applicable low and medium complexity laboratories. In the present study, we developed compared different techniques using strains genetically identified as carbapenemase-producing. All tested methods detected presenc...

Phenotypic assays measure the susceptibility of HIV-1 to antiretroviral drugs, and are used to guide treatment regimen choices. Analyses of baseline predictors of virological failure support the use of a 0.4-fold cutpoint for defining increased susceptibility (hypersusceptibility) to efavirenz in the PhenoSense HIV assay. The enhancement of the virological activity of efavirenz, associated with...

Material and methods MM pts received an induction therapy with IPI 10 mg/kg every 3 weeks (Q3W) for four doses and FTM 100 mg/m2 weekly for 3 weeks. Peripheral blood lymphocytes (PBMC) and sera were collected at baseline, wk12, and wk24 to perform phenotypic and functional T cell assays, and to investigate humoral responses against a panel of tumorassociated antigens (TAAs) and soluble NKG2D li...

A chemical genetics approach has been applied in the screening of yeast deletants strains with a pool of morpholine-derived compounds in order to identify candidate small molecules able to produce phenotypic effects on yeast cells. The analysis of the effects of structurally diverse molecules towards cell growth rate in both exponential and stationary phases provides a tool to select candidate ...

Vulvovaginal Candidiasis (VVC) is a fungal infection caused by Candida spp. of the genital mucosa that still health concern for women.This first-of-its-kind investigation on isolation dubliniensis, from cervical-vulvovaginal patients in Erbil hospitals. The study was carried out and identification which performed using phenotypic molecular approaches. A total one hundred samples vaginal swabs (...

A duplex PCR assay targeting the ail and 16S rRNA genes of Yersinia enterocolitica was developed to specifically identify pathogenic Y. enterocolitica from pure culture. Validation of the assay was performed with 215 clinical Yersinia strains and 40 strains of other bacterial species. Within an assay time of 4 h, this assay offers a very specific, reliable, and inexpensive alternative to the co...

Methods: The studied population included 59 MDRAB strains previously genotyped by PFGE and multilocus sequence typing. The search for the ADC (blaADC), CHO (blaOXA-51-like, blaOXA-23-like, blaOXA-40-like and blaOXA-58-like) and MBL (blaIMP, blaVIM, blaSIM-1, blaGIM-1, blaSPM-1 and blaNDM-1) genes, and for the ISs (ISAba1, ISAba2, ISAba3, ISAba4 and IS18) was done by PCR assays. The phenotypic p...