Understanding of interactions between cells and biomaterials is a huge parameter for improving tissue engineering and regenerative medical fields. Many different materials have already been tested (including calcium phosphate ceramics) and it has been established that surface characteristic is a parameter that influences cell responses. The aim of this work was to characterize calcium phosphate...

Calcium phosphate ceramics are widely used as bone substitutes since they are biocompatible and bioactive. Given that their chemical composition is close to natural bone, calcium phosphate ceramics are promising bone substitute materials in orthopaedics, maxillofacial surgery and dentistry. Hydroxyapatite (HA) and tricalcium phosphate (TCP) are the most commonly used calcium phosphates, because...

This study was conducted to develop novel ceramic bone substitute that resembles the autologous bone behavior when used as graft material. Solid-state reaction at 1100°C was performed to synthesize β-tricalcium phosphate (β-TCP) and biphasic calcium phosphate (BCP). The ceramics were further analyzed to characterize phase composition, microstructural properties, cytocompatability and then chall...

The usual procedure for the preparation of succinate oxidase involves the extraction of washed heart muscle with a mildly alkaline phosphate solution. A turbid dispersion is thus obtained which is resistant to precipitation by a low centrifugal force and which contains at least cytochrome oxidase, cytochromes a, 5, c, and a flavoprotein. This enzyme preparation, besides catalyzing the oxidation...

Calcium phosphate (Ca-P) coatings on zirconia bone implants have a great potential to improve the osseointegration of already existing ceramic implants, owing to their bone-bonding ability and high osteoconductive characteristics. In our study, we have prepared three different kinds of Ca-P coatings, namely the octacalcium phosphate (OCP), hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP)...

We report the 5.008-Mbp assembled draft genome sequence of Desulfotignum phosphitoxidans strain FiPS-3 (DSM 13687), which gains metabolic energy from the oxidation of phosphite to phosphate. Its genome provides insights into the composition and architecture of the phosphite-utilizing and energy-transducing systems required to live with phosphite as electron donor.

Extracellular high-mol.-wt material was separated from liquid cultures of Staphylococcus epidermidis. This material contained protein c. 20% w/w and polysaccharide c. 80% w/w. The polysaccharide was isolated by gel and ion-exchange chromatography and contained glycerol phosphate, glucose, N-acetylglucosamine, and D-alanine. Cell-wall teichoic acid was isolated from strain RP-62A and had a simil...

Cows’ milk contains inorganic calcium phosphate as a tightly-bound component of the casein micelles. The composition and chemical properties of this calcium phosphate have been studied for many years. Although the Ca/P ratio is similar to that found in hydroxyapatite, the use of other methods of characterization is desirable, since the various crystalline and amorphous forms of inorganic calciu...

Wolfe-Simon et al. (Research Articles, 3 June 2011, p. 1163; published online 2 December 2010) argued that the bacterial strain GFAJ-1 can vary the elemental composition of its biomolecules by substituting arsenic for phosphorus. Although their data show that GFAJ-1 is an extraordinary extremophile, consideration of arsenate redox chemistry undermines the suggestion that arsenate can replace th...

The peculiarities of K2Gd(PO4)(MoO4) single crystal growth from combined phosphate-molybdate melts have been investigated. formation regions GdPO4, KGd(MoO4)2 and their cocrystallization fields with the mixed anionic compound potassium – gadolinium established. IR spect­roscopy showed that in all compounds molybdenum phosphorus are a tetrahedral environment, phase composition was confirmed by X...