The enzymatic synthesis of inositol monophosphatide has been described in reports by Agranoff et al. (l), Thompson et al. (2), and Paulus and Kennedy (3, 4). An enzymatic reaction in which free inositol is incorporated into inositol phosphatide, stimulated by the addition of any of a number of cytidine nucleotides, including cytidine 5’-monophosphate and cytidine diphosphate choline, was fnst o...

Inositol is usually classified as an essential vitamin for most animals, and is recognised as a part of the B-complex vitamins. Among all other inositol isomer forms, myo-inositol possesses biological activity. It is found in the brain, skeletal, heart, and main reproductive tissues and exists as a structural component of phosphatidylinositol in biological cell membranes. Myo-inositol, also act...

Background and Aims: The aim was to determine the role of dominant serovars of Listeria monocytogenes (L. monocytogenes) in spontaneous abortions, using isolation methods and polymerase change reaction (PCR). Materials and Methods: A total of 258 samples comprising of placental tissue, vaginal swabs and blood were collected from 123 patients with spontaneous abortion. L. monocytogenes was iden...

Abstract Objective We aimed to investigate the therapeutic effects of Moringa oleifera leaf extracts on osteogenic induction rat bone marrow mesenchymal stem cells (BMSCs) following peroxidative damage and explore underlying mechanisms. Methods Conditioned medium was used induce differentiation BMSCs, which were treated with H 2 O , extracts-containing serum, or phosphatidyl inositol-3 kinase (...

A single-cell assay was developed to measure the activation of phosphoinositide 3-kinase (PI3K) using microanalytical chemical separations and a fluorescently labeled lipid substrate. Phosphatidyl-inositol 4,5 bisphosphate labeled on its acyl chain with Bodipy fluorescein (Bodipy Fl PIP(2)) was utilized as a substrate for both in vitro and cell-based assays. Detection limits for the substrate a...