human cytomegalovirus is one of the most common pathogenic viruses all over the world. in congenital infection leads to neurologic severe disorders and even death of fetus and in individuals with immunosuppression may also cause severe clinical symptoms. multiple evidence indicate that among several strategies, epitope-based vaccine (evs) that can induce both humoral and cellular immunity respo...

background:in the recent decade, the reverse genetics method has been broadly used for rescue of negative-stranded rna viruses from cdna or viral minigenomes. this technique has been applied to study different steps in virus replication and virus-host interactions. reverse genetics could also be implemented for design of new vaccines. the t7 rna polymerase activity as well as virus (nucleocapsi...

A novel peptide substrate for adenosine 3',5'-cyclic monophosphate-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37), Leu-Arg-Arg-Trp-Ser-Leu-Gly, was synthesized. Phosphorylation of the peptide causes a 20% increase in the peptide fluorescence intensity at 358 nm. Values of Km and kcat for the phosphorylation reaction at pH 7.0 (25 degrees C), were determined to be 2.7 +/-...

1. The subcellular distribution of the phosphoprotein fraction of grey matter from guinea-pig brain has been determined. Phosphoproteins occurred in all fractions of the tissue, the mitochondrial fraction containing 29%, supernatant 26%, microsomal fraction 23% and nuclear fraction 15%. The quantity of phosphoproteins per unit quantity of protein was highest in the microsomal fraction and lowes...

A rapid, manyfold increase in phosphorylation of cardiac microsomes consisting primarily of sarcoplasmic reticulum was seen when these membranes were incubated in the presence of a bovine cardiac adenosine 3’:5’-monophosphate (cyclic AMP)-dependent protein kinase (protein kinase) and cyclic AMP. Over 85 % of the 3*P associated with membrane protein under similar conditions was identified cs pho...

It is now well established that the synthesis of the major protein constituents of milk takes place exclusively in the mammary gland, utilizing as building materials the free amino acids of the blood (1). The research of Aten and Hevesy (2), confirmed subsequently by other workers (3, 4), leaves little room for doubt that the precursor of the casein phosphorus is the inorganic phosphorus of the...

BACKGROUND Human epidermal growth factor receptor (HER) downstream signaling kinases have important effects on tumor response to anti-HER monoclonal antibodies and tyrosine kinase inhibitors. We validated an assay that uses phosphoprotein arrays for measurement of HER downstream signaling functionality in breast carcinomas. METHODS Using the Bio-Plex(R) phosphoprotein array (BPA), we performe...

The rapid appearance of radioactivity in the phosphoprotein fraction of tissues after exposure to inorganic radiophosphate was first noted by Davidson et al. (1). This finding was subsequently confirmed in several laboratories for a variety of cell types (2-5). The physiological function of phosphoproteins is obscure, however, since no direct evidence is yet available for their role in cell met...