A A23187 (Ca2þ ionophore), 215, 216 Acetamidoiminodiacetic acid (ADA), affinity for Ca2þ, 537t Acetoxymethyl (AM) esters of Ca2þ indicators, 408. See also specific indicators Acids disposal of, 573 safe handling of, 572, 575 Acridine orange, 521–523 ACSF for calcium imaging (recipe), 149, 152 Action potentials, back propagating, 142, 142f Activation of Ca2þ-dependent ion channels, 79–84 ADA (ac...

of a dissertation at the University of Miami. Light emitting molecules are an indispensable part of detection and reporting in many fields and are employed in a variety of biomedical applications. Bioluminescent light, or living light, from bioluminescent proteins in particular has many beneficial characteristics, including their lack of a need for an outside excitation source and detection at ...

Arthropod hormone receptors are potential targets for novel pesticides as they regulate many essential physiological and behavioral processes. The majority of them belong to the superfamily of G protein-coupled receptors (GPCRs). We have focused on characterizing arthropod kinin receptors from the tick and mosquito. Arthropod kinins are multifunctional neuropeptides with myotropic, diuretic, an...

The source and sinks for the intracellular calcium released during fertilization were examined in single eggs from the sea urchin, Arbacia punctulata. Single eggs were microinjected with the calcium photoprotein, aequorin. The calcium-aequorin luminescence was measured with a microscope-photomultiplier or observed with a microscope-image intensifier-video system. In the normal egg a propagated ...

tations of GD [12] . TSHR bioassays show outstanding features. The biological activity of specific immunoglobulins is directly assessed on a fully functional TSHR holoreceptor expressed on intact live cells, a platform that is easily adaptable and tailored to detect Ab of specific function. The TSHR protein structure can be bioengineered and stably expressed in cell lines with protocols optimiz...

tations of GD [12] . TSHR bioassays show outstanding features. The biological activity of specific immunoglobulins is directly assessed on a fully functional TSHR holoreceptor expressed on intact live cells, a platform that is easily adaptable and tailored to detect Ab of specific function. The TSHR protein structure can be bioengineered and stably expressed in cell lines with protocols optimiz...

A molecularly tagged form of calreticulin (CR), a low affinity-high capacity Ca 2+ binding protein that resides in the ER lumen, was transiently transfected into HeLa cells to specifically modify the Ca 2÷ buffering capacity of the intracellular Ca 2÷ stores. Fluorescence and confocal microscope immunocytochemistry revealed the tagged protein to be expressed by over 40% of the cells and to over...

tations of GD [12] . TSHR bioassays show outstanding features. The biological activity of specific immunoglobulins is directly assessed on a fully functional TSHR holoreceptor expressed on intact live cells, a platform that is easily adaptable and tailored to detect Ab of specific function. The TSHR protein structure can be bioengineered and stably expressed in cell lines with protocols optimiz...

tations of GD [12] . TSHR bioassays show outstanding features. The biological activity of specific immunoglobulins is directly assessed on a fully functional TSHR holoreceptor expressed on intact live cells, a platform that is easily adaptable and tailored to detect Ab of specific function. The TSHR protein structure can be bioengineered and stably expressed in cell lines with protocols optimiz...

tations of GD [12] . TSHR bioassays show outstanding features. The biological activity of specific immunoglobulins is directly assessed on a fully functional TSHR holoreceptor expressed on intact live cells, a platform that is easily adaptable and tailored to detect Ab of specific function. The TSHR protein structure can be bioengineered and stably expressed in cell lines with protocols optimiz...