A study was conducted to investigate the effects of thermostable phytase supplementation on the growth performance and nutrient digestibility of broilers for 42 d. 1-day-old Arbor Acres chicks with similar initial body weight were randomly divided into 3 treatments consisting 6 pens of 20 chicks per pen. The control group was given maize-soybean meal basal diet and the other two groups were fed...

Two studies were conducted to determine the true P digestibility (TPD) of black-eyed pea (BEP) and peanut flour (PNF) and the TPD response to phytase supplementation using the regression method. Sequential diets containing 115, 230, or 345 g of BEP/kg (experiment 1) and 115, 230, or 345 g of PNF/kg (experiment 2) without or with 1,000 units of phytase/kg were formulated. Chromic oxide was added...

Background: Old laying hens are more prone to dietary calcium (Ca) and phosphorus (P) deficiencies as  they absorb Ca and P less efficiently than younger hens. OBJECTIVES: In a 2×2 factorial design, the influence of diets with two levels of available phosphorus (AP) and phytase enzyme on the laying performance, egg quality and tibia bone characteristics of laying hens at their late 2nd producti...

The methodology for the preparation of cross-linked enzyme aggregates (CLEAs) and their application as highly effective, recyclable biocatalysts in a wide variety of transformations is reviewed. The method constitutes a combination of enzyme purification and immobilization into a single operation and allows for the use of relatively crude enzyme preparations. The resulting CLEAs have excellent ...

Traditional supplemental dietary phytase loses activity during steam pelleting. The thermal tolerance and bioefficacy of a phytase product with a thermoprotective coating [coated phytase (C-phytase)] was compared in mash and pelleted diets to a traditional, uncoated phytase (U-phytase) added to a negative control (NC) diet, formulated with reduced dietary Ca and P, and compared with a corn-soyb...

Three experiments were conducted to test the hypotheses that pigs fed diets that are equal in digestible P will perform equally regardless of the concentration of total P in the diets, and that the addition of microbial phytase, distillers dried grains with solubles (DDGS), or a combination of phytase and DDGS will result in a reduction in P excretion. In Exp. 1, a P-free diet and 6 diets conta...

Two studies with growing pigs were conducted using the regression analysis technique to estimate the true digestibility of P (TPD) in canola (CM) and soybean (SBM) meals, and quantify the effect of microbial phytase on TPD of CM and SBM. In each study, 48 (Exp. 1) or 36 (Exp. 2) 17-kg barrows were assigned to 6 dietary treatments arranged in a 3 x 2 factorial of 3 graded levels of CM (Exp. 1) o...

During germination, maize seedlings express a phytase able to hydrolyse the large amount of phytin stored in the dry seed. Previous studies allowed purification and characterization of this enzyme as a homodimer of 38 kDa subunits [Laboure, Gagnon and Lescure, Biochem. J. (1993) 295, 413-419]. In the present work, an antibody against the purified maize phytase has been used to screen a maize se...

Five barrows of approximately 45 kg BW, fitted with post-valvular T-cecum cannulas at the ileo-cecal junction, were assigned randomly to five treatments (5 x 5 Latin square design) to assess the effect of microbial phytase and feeding regimen (frequency and level) on the apparent digestibilities (total tract [ATTD] and ileal [AID]) and retention of nutrients. A corn-tapioca-soybean meal diet of...

Animal nutrition industry demands for phytases with high specific activity and stability under high temperature conditions (feed pellet production) and under acidic conditions (monogastric animals), for this reason continues to stimulate the search for new enzyme sources [1]. Most of scientific work has been focused on Aspergillus spp. phytases due to their biochemical properties found [2]. An ...