background: platelets rapidly lose their qualities usually after 5 day of storage. different standard methods have been recommended to check the quality of platelets during storage which some of them show better correlation with other quality markers during storage. the purpose of this study was to demonstrate if platelet factor 3 (pf3) assay could be an indicator of storage lesion and provide ...

The therapeutic efficacy of mesenchymal stromal cells (MSCs) has been shown to rely on their immunomodulatory and regenerative properties. In order obtain sufficient numbers for clinical applications, MSCs have be expanded ex vivo. Expansion media with xenogeneic-free (XF) growth-promoting supplements like human platelet lysate (PL) or serum- (SF/XF) formulations established as safe efficient, ...

objective: non:union: is defined as a minimum of 9 months since injury without any visible progressive signs of healing for 3 months. recent literature has shown that the application of mesenchymal stromal cells is safe, in vitro and in vivo, for treating long bone non:union:. the present study was performed to investigate the safety of mesenchymal stromal cell (msc) implantation in combination...

background: immune thrombocytopenia is a hematologic disorder characterized by low platelet count and variable bleeding manifestations due to immune-mediated platelet destruction and/or suppression of platelet production. this study was performed to evaluate characteristics and clinical presentations of adult patients with immune thrombocytopenia and to explore the clinical value of platelet an...

BACKGROUND Standard protocols are lacking for the preparation of platelet lysates (PL) as an alternative to using fetal bovine serum as a cell culture supplement. This study aimed to establish optimum conditions for preparing PL for use in cell cultures. METHODS Cell density in three pooled platelet concentrates (PC) were adjusted to 1×10(12)/L and 2×10(12)/L. PL was prepared from PC by 1 to ...

Revascularisation is a key step for tissue regeneration and complete organ engineering. We describe the generation of human platelet lysate gel (hPLG), an extracellular matrix preparation from human platelets able to support the proliferation of endothelial colony forming cells (ECFCs) in 2D cultures and the formation of a complete microvascular network in vitro in 3D cultures. Existing extrace...

Aim: Despite intensive research during the last decade, it remains challenging to prepare extracellular vesicles (EVs) of high purity, especially from primary body liquids or protein-rich conditioned media. For now, time-consuming combinations at least two orthogonal methods, e.g., density and size separation, are required enrich EVs often expense processing time. Therefore, novel technologies ...

The regenerative potential declines upon aging. This might be due to cell-intrinsic changes in stem and progenitor cells or to influences by the microenvironment. Mesenchymal stem cells (MSC) raise high hopes in regenerative medicine. They are usually culture expanded in media with fetal calf serum (FCS) or other serum supplements such as human platelet lysate (HPL). In this study, we have anal...

BACKGROUND The purpose of this study was to investigate the efficacy and safety of autologous platelet lysate (APL) local injections in reducing pain and improving function in patients with refractory lateral epicondylitis. METHODS A total of 56 patients with refractory lateral epicondylitis were enrolled in this study. All the patients received three injections in one course of treatment. Su...

Meniscal lesions, depending on their severity and location, sometimes heal spontaneously or may remain asymptomatic. Osteoarthritis (OA) is frequently associated with both symptomatic and asymptomatic meniscal defects [16]. Treatment of meniscal tears by surgical interventions, including resection, refixation/suturing, scaffold implants and allografts, is the clinical standard; however, there i...