Cells closely coordinate cell division with chromosome replication and segregation; however, the mechanisms responsible for this coordination still remain largely unknown. Here, we analyzed the spatial arrangement and temporal dynamics of the 9.1 Mb circular chromosome in the rod-shaped cells of Myxococcus xanthus. For chromosome segregation, M. xanthus uses a parABS system, which is essential,...

valois (vls) was identified as a posterior group gene in the initial screens for Drosophila maternal-effect lethal mutations. Despite its early genetic identification, it has not been characterized at the molecular level until now. We show that vls encodes a divergent WD domain protein and that the three available EMS-induced point mutations cause premature stop codons in the vls ORF. We have g...

Proteasome-mediated protein degradation is a key regulatory mechanism in a diversity of complex processes, including the control of cell cycle progression. The selection of substrates for degradation clearly depends on the specificity of ubiquitination mechanisms, but further regulation may occur within the proteasomal 19S cap complexes, which attach to the ends of the 20S proteolytic core and ...

We discuss the role of nucleon and its excited state poles in the twist expansion of the nucleon structure function moments. We find that the nucleon pole contribution was overestimated in previous analyses by a factor of two. Inclusion of this missing factor makes the duality appear for all moments at least down to Q2 = 1 GeV2. For resonance poles the time reversal invariance together with uni...

Various proteases in the brain contribute to ischemic brain injury. We investigated the involvement of the asparaginyl endopeptidase legumain after experimental stroke. On the basis of gene array studies and in situ hybridizations, we observed an increase of legumain expression in the peri-infarct area of rats after transient occlusion of the middle cerebral artery (MCAO) for 120 mins with a ma...

This paper derives new formulations for designing dominant pole placement based proportionalintegral-derivative (PID) controllers to handle second order processes with time delays (SOPTD). Previously, similar attempts have been made for pole placement in delay-free systems. The presence of the time delay term manifests itself as a higher order system with variable number of interlaced poles and...

The authors show how continuous-time active-RC filters can be implemented in CMOS by replacing all resistors by MOSFETs operating in the linear region. As an example, a 24MHz activeMOSFET-C single-amplifier biquadratic lowpass filter with a pole-Q of 3 implemented in a 0.6μm CMOS process is discussed. By comparing measurements of a test chip, simulations and calculations, the following conclusi...

Placement of the division site at midcell in Escherichia coli requires the MinE protein. MinE acts by imparting topological specificity to the MinCD division inhibitor, preventing the inhibitor from acting at the midcell site while permitting it to block division at other unwanted sites along the length of the cell. It was previously shown that MinE assembled into a ring structure that appeared...

The exact frame-dragging (or Lense-Thirring (LT) precession) rates for Kerr, Kerr-Taub-NUT (KTN) and Taub-NUT spacetimes have been derived. Remarkably, in the case of the ‘zero angular momentum’ Taub-NUT spacetime, the frame-dragging effect is shown not to vanish, when considered for spinning test gyroscope. In the case of the interior of the pulsars, the exact framedragging rate monotonically ...

panniculitis. Result: The most common clinical manifestation was a depressed patch on upper arm. There was 46oh and l3o/o positivity in the ANA and anti-ds DNA test, respectively. His topatho log ica l ly , a l l spec imens revealed lobu lar panniculitis and the majority of cases presented epidermal atrophy and dermal infiltration. Immunohistochemistry showed a predominance of T lymphocytes. Al...