The ligand N alpha, B1-(6-biotinylamido)hexanoyl-insulin was attached noncovalently to Sepharose 4B immobilized succinoylavidin to form an insulin-affinity resin. This resin was used to isolate highly purified insulin receptor from human placental tissue by a four step process involving (i) preparation of a crude membrane fraction, (ii) solubilization with Triton X-100, (iii) wheat germ aggluti...

An IgM monoclonal antibody (AGP3) against polyethylene glycol (PEG) was used to assay PEG-modified proteins by ELISA. PEG-modified beta-glucuronidase could be measured at concentrations as low as 15 ng/mL, corresponding to 750 pg (1.8 fmol) of conjugate. This ELISA should be generally applicable to all PEG-modified proteins because AGP3 binds the backbone of the PEG chain independent of the lin...

Copolymers between polylactic acid and polyethylene glycol, also alganic glycol were synthesized disocynate (MDI) prepared characterized. Swelling behaviors loss of weight these polyurethanes studied by immersion the gels in various buffer solutions. Particularly polyurethane foam showed highest EWC (equilibrium water constant) timedependent pH-dependent swelling.

Environmental stresses specially drought, play an important role in decreasing plant growth, particularly during germination in dry and semi dry area. To considering the effect of drought stress caused by polyethylene glycol on germination and characteristics of 2 spices of Sesamum indicum, we had done factorial and complete accidental plot with 4 treatments and 3 times repetition. Experimental...

Nano-sized γ-Al2O3 was synthesized successfully by a simple method using Al2(SO4)3·18H2O as aluminium source and in the presence of polyethylene glycol (PEG 2000). FTIR spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM) were used to characterize the structure and morphology of synthesized powder. The results showed that PEG playe...

Cationic waterborne polyurethane as a new dispersing polymer was synthesized by using relatively hydrophilic polyols. Dispersing cationic polyurethane (DCPU) nanocomposites were prepared using isophorone diisocyanate (IPDI), polyethylene glycol (PEG) with different molecular weights (Mn=200, 400, 600,and1000 g/mol), N-methyl diethanolamine (MDEA), dibutyltin dilaurate (DBTDL) and different perc...

Assessing the tolerance of medicinal plants is important for planting them in drought areas. Arginine is a growth regulator and its role in plants’ tolerance to environmental stresses such as drought has been investigated. To evaluate the protective effects of arginine against osmotic stress induced by polyethylene glycol in ajwain (Trachyspermum ammi) seedlings, an experiment was conducted as ...

We compared six methods for isolating high-density lipoproteins from normal and lipemic serum. Cholesterol was determined by two different enzymic methods. The values for high-density lipoprotein cholesterol obtained with two (centrifugation-speed) modifications of the heparin/Mn2 method and with the phosphotungstate/Mg2t the dextran sulfate/Mg2, and the polyethylene glycol methods were compare...

OBJECTIVE To determine the efficacy, tolerance, and safety of oral sodium phosphate compared with polyethylene glycol in patients with chronic constipation. MATERIAL AND METHODS From May 2007 to October 2007, 100 patients with chronic constipation were prospectively randomized into two groups for colonoscopy. Group A (n=49) received sodium phosphate before colonoscopy, while Group B (n=51) re...

BACKGROUND The objective of this study was to prepare, characterize, and evaluate a folate-modified self-microemulsifying drug delivery system (FSMEDDS) with the aim to improve the solubility of curcumin and its delivery to the colon, facilitating endocytosis of FSMEDDS mediated by folate receptors on colon cancer cells. METHODS Ternary phase diagrams were constructed in order to obtain the m...