Mutations in the adenomatous polyposis coli (Apc) tumor suppressor gene represent the primary genetic defect in colon carcinogenesis. Apc+/- mouse models exhibit pre-invasive small intestinal adenomas. Cell culture models exhibiting Apc defects in the colon and quantifiable cancer risk provide a novel clinically relevant approach. The tumor-derived Apc-/- colonic epithelial cell line 1638N COL-...

Pr1−xCaxBa2Cu3O7−δ(x ≈0.5) thin films have been grown on SrTiO3 and YSZ substrates by the pulsed laser ablation. The substrate temperature dependence of orientation and superconducting poperties were systematically studied. Good quality c and a-axis orientated films can be obtained on SrTiO3 via changing the substrate temperature solely. On YSZ, films with good c-axis orientation can be grown, ...

The ligand binding domain of the low density lipoprotein receptor consists of seven cysteine-rich repeats of approximately 40 amino acids each. These repeats, which are located at the NH2 terminus of the protein, are homologous to sequences in complement components C8 and C9. To determine the role of the first repeat (amino acids 2-42), we prepared two plasmids containing expressible low densit...

Sunflower leaf discs floated on a solution containing aspirin (acetylsalicylic acid) produced a set of new proteins extractable at pH 5.2 and excreted into the intercellular space. More than 80% of the proteins found in the intercellular fluids of induced leaf discs have been identified as pathogenesis-related (PR) proteins by their immunological relationship with tobacco PR proteins. Members o...

Colorado Denver. The inducible head-and-neck specific knockout system consists of two mouse lines, the K14.CrePR1 or K5.Cre*PR1 mice, in which Cre recombinase can be activated in head-and-neck epithelia by RU486 (1, 2), and the Smad4f/f mice, in which the Smad4 gene is floxed (3). These mouse lines were crossbred to generate compound mice, allowing for homozygous Smad4 deletion (Figure S1A). Li...

A major class of plant disease resistance (R) genes encodes leucine-rich-repeat proteins that possess a nucleotide binding site and amino-terminal similarity to the cytoplasmic domains of the Drosophila Toll and human IL-1 receptors. In Arabidopsis thaliana, EDS1 is indispensable for the function of these R genes. The EDS1 gene was cloned by targeted transposon tagging and found to encode a pro...

The basal defenses important in curtailing the development of the phloem-feeding silverleaf whitefly (Bemisia tabaci type B; SLWF) on Arabidopsis (Arabidopsis thaliana) were investigated. Sentinel defense gene RNAs were monitored in SLWF-infested and control plants. Salicylic acid (SA)-responsive gene transcripts accumulated locally (PR1, BGL2, PR5, SID2, EDS5, PAD4) and systemically (PR1, BGL2...

Plants have evolved strong innate immunity mechanisms, but successful pathogens evade or suppress plant immunity via effectors delivered into the plant cell. Hyaloperonospora arabidopsidis (Hpa) causes downy mildew on Arabidopsis thaliana, and a genome sequence is available for isolate Emoy2. Here, we exploit the availability of genome sequences for Hpa and Arabidopsis to measure gene-expressio...

Rashid I1, Sharma S2, Kamboj G2, Deshwal PR1, Akhilesh KJ2, Tiwari P*3 and D’Cruz S4 Author Affiliations 1Ph.D. Scholar, Department of Pharmacy Practice, National Institute Pharmaceutical Education Research (NIPER), Punjab, India 2M. Pharm. 3Prof. & Head, India. 4Prof General Medicine, Government Medical College Hospital, Chandigarh, Received: January 27, 2022 | Published: February 11, Correspo...