Two patients with low lactate dehydrogenase (LD) activity were discovered during healthcare examinations and were found to be homozygous for LD-H (heart) subunit deficiency by electrophoretic isoenzyme analysis of serum and erythrocyte hemolysate. The molecular nature of the genetic mutations was characterized by amplification by the polymerase chain reaction and DNA sequencing. In one case, a ...

The study aimed to translate and to adapt a version of the Premature Infant Pain Profile into the Brazilian Portuguese language. This is a cross-sectional and methodological study for the validation of a translated version of a tool. The process was conducted in four stages: initial translation, synthesis, back-translation, and analysis by experts. Four independent versions of the instrument tr...

prevention and treatment of the rickets of prematurity is an important aspect of the care of preterm infants. the purpose of this study was to compare the prophylactic effects of different doses of vitamin d on the clinical, biochemical and radiological indices of the rickets of prematurity. in a randomized clinical trial, 68 premature infants (<38 weeks) with birth weight under 2000 g, randoml...

introduction: the acquisition of speech and language constitutes a dynamic part of two-year-old children’s growth. expressive lexicon is a key indicator of language ability in these children. premature birth, birth before gestational week 37, is one of the most common risk factors associated with learning skill development. a review of literature suggests inconsistent results on the relationshi...

  In this article the possible effects of the translation technologies on the critical thinking and translation ability of the students of the Translation Studies would be studied through a pre-experimental method of research. The participants of this research were thirty one Iranian senior Translation Studies students at the age group of 20-26 who were selected based on their age, educational...

We have analyzed a cloned beta O-thalassemia (beta O-thal) gene from a patient doubly heterozygous for hemoglobin Lepore and beta O-thalassemia. Studies of 3H-uridine incorporation into beta-globin mRNA in this patient's erythroblasts suggested an intranuclear defect in both beta and Lepore (delta beta) mRNA synthesis, as did S1 nuclease analysis of nuclear RNA. However, the nucleotide sequence...

The generation of peptides containing N-methyl amino acids (N-Me AAs) by in-vitro translation is of interest as a potential route to the directed evolution of pharmaceutically useful modified peptides. However, N-Me AAs are suboptimal substrates for the translational apparatus, and as a result, premature termination of translation can occur or incorrect amino acids can be misincorporated in pla...