cloning could be achieved by using this quick, alternative protocol. Not only does this Na3VO4-inhibition procedure save time, but also note that in this protocol the adjustment of vector-to-insert ratio in the ligation reaction is not necessary. To ligate with the CIAP/Na3VO4-treated vector (ca. 50 ng), we recommend using as much excess insert as is practical. Eliminating the quantitation step...

Formation of compact particles from linear DNA-anthracycline complexes is accompanied by appearance of intense bands in the CD spectra in the region of absorption of DNA bases (UV-region) and in the region of absorption of anthracycline chromophores (visible region). The intense (positive or negative) bands in the region of anthracycline absorption demonstrate an ordered helical location of ant...

Background: despite the fact that the genetic basis of schizophrenia has been intensively studied for more than two decades, our contemporary knowledge in this field is rather fractional, and a substantial part of it is still missing. The aim of this review article is to sum up the data coming from genome ‐wide association genetic studies in schizophrenia, and indicate prospective directions of...

an eight ‐month ‐old male child was admitted with weakness and swelling in the feet. paraparesis and bilateral lower extremity edema were present in the neurological examination. thoracic MRi showed an intradural intramedullary mass 61 × 11 mm in size in the t5–t10 levels. laminotomy between the t5–t10 vertebrae was performed. a mass with smooth borders was separated from most of the neural tis...

The reactivating and therapeutic efficacy of two combinations of oximes (HI ‐6 + trimedoxime and HI ‐6 + K203) was compared with the effectiveness of antidotal treatment involving single oxime (hi ‐6, trimedoxime, K203) using in vivo methods. In vivo determined percentage of reactivation of cyclosarin ‐inhibited blood and tissue acetylcholinesterase in poisoned rats showed that the reactivating...

COPD is a chronic multisystem disorder with various co morbidities. Smoking is a major risk factor. It’s etiology and multisystem effects may contribute to depression and anxiety. Interplay of various factors along with gender, socioeconomic, educational and marital status also do contribute to depression and anxiety in COPD. Unfortunately, Evaluation of anxiety and depression are often neglect...

Many reports highlight biological responses of crop plants after non-ionizing electromagnetic radiation (EMR) exposure based on the phenotypic and physiological levels. So, this study aimed to estimate genetic alterations in proteins, isozymes, DNA banding patterns as well extent nuclear damage economic corn (Zea mays) under stress EMR using accurate reliable bioassays like sodium dodecyl sulfa...

We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected by hybridization with 32P-labeled DNA probes followed by autoradiography. Thi...

introduction: different isolates of human specifies, giardia intestinalis, also known as giardia lambia, have been shown to vary in sensitivity to drug, isoenzyme pattern, virulence and infectivity, antigens and restriction enzyme pattern. material and methods: using system that distinguishes sequence differences in the metabolic enzyme tpi, dna extracted from trophozoite that culture in modifi...