Hydrogen fuel is renewable, efficient and clean, and fermentative bacteria hold great promise for its generation. Here we use the isogenic Escherichia coli K-12 KEIO library to rapidly construct multiple, precise deletions in the E. coli genome to direct the metabolic flux towards hydrogen production. Escherichia coli has three active hydrogenases, and the genes involved in the regulation of th...

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There is currently substantial interest in utilizing eukaryotic algae for the renewable production of several bioenergy carriers, including starches for alcohols, lipids for diesel fuel surrogates, and H2 for fuel cells. Relative to terrestrial biofuel feedstocks, algae can convert solar energy into fuels at higher photosynthetic efficiencies, and can thrive in salt water systems. Recently, the...

The inherent safety, convenience, availability, nutritional content, aesthetic appeal, and variety that typify our food supplies are a hallmark of modern life, but this was not always the case. For the last 100 years we have been witnessing dramatic advances in the scientific understanding and engineering techniques that increase agricultural production and allow for the commercial-scale produc...

BACKGROUND Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium's GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications derived from L-tryptophan. RESULTS Corynebacterium glutamicum ATCC 21850 that co...

Hydrogen production by green algae has significant advantages over other photobiological systems: ATP production is not required, high theoretical efficiencies are possible, and water is used directly as the source of reductant without the need to produce biomass or to store intermediary carbon metabolites. However, algal H2 photoproduction is sensitive to O2, a coproduct of photosynthesis, and...

The pharmaceutically important anticancer drugs etoposide and teniposide are derived from podophyllotoxin, a natural product isolated from roots of Podophyllum hexandrum growing in the wild. The overexploitation of this endangered plant has led to the search for alternative sources. Metabolic engineering aimed at constructing the pathway in another host cell is very appealing, but for that appr...

Biotechnology can be broadly defined as the application of scientific procedures to processes that use living organisms or genetic material derived from them to develop innovative products. This definition covers a broad spectrum of genetic manipulations. A refined definition is that for genetic engineering which describes the introduction of genes into organisms by direct transfer of DNA inclu...

mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. several recombinant protein systems have been introduced to the market, most of which are under clinical development. in spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...