Poliovirus genomic RNA replication, protein translation, and virion assembly are performed in the cytoplasm of host cells. However, this does not mean that there is no relationship between poliovirus infection and the cellular nucleus. In this study, recombinant fluorescence-tagged poliovirus 3CD and 3C0 proteins were shown to be expressed mainly in the cytoplasm of Vero cells in the absence of...

Hepatitis C virus (HCV) infection is a leading cause of chronic liver diseases worldwide, but treatment options are limited. Basic HCV research required for vaccine and drug development has been hampered by inability to culture patient isolates, and to date only the JFH1 (genotype 2a) recombinant replicates spontaneously in hepatoma cells and releases infectious virus. A JFH1 chimera with the 5...

Penicilliurn expamum grown in a medium with rice husk as a carbon source produced an extracellular protease. The protease enzyme was isolated from culture broth by fractionation with acetone and column chromatography on Sephadex G- 100 and DEAE A-50. The protease enzyme was purified about 17.47 fold, with a recovery of 14%. The purified protease was homogenous on SDS polyacrylarnide disc ge...

conclusions in this study, we isolated b. licheniformis producing an organic solvent-stable protease from oil-contaminated soil. the protease was stable and active in various organic solvents. by purification, the protease could be used as a biocatalyst for organic solvent-based enzymatic synthesis. results according to 16srdna sequencing, strain isa9 was identified as a new strain of bacillus ...

htlv-1 and hiv-1 are two major causes for severe t-cell leukemia disease and acquired immune deficiency syndrome (aids). htlv-1 protease, a member of aspartic acid protease family, plays important roles in maturation during virus replication cycle. the impairment of these proteases results in uninfectious htlv-1virions.similar to hiv-1protease deliberate mutations that confer drug resistance on...

Background: Alkaline proteases is the important group of enzymes having numerous industrial applications including dairy food formulations. Objectives: The current study deals with the purification and characterization of an alkaline serine protease produced by Geotrichum candidum QAUGC01, isolated from indigenous fermented milk product, Dahi.<br...

background: fasciola hepatica is one of the most important helminthes parasites and triclabendazole (tcbz) is routinely used for treatment of infected people and animals. secreted protease enzymes by the f. hepatica plays a critical role in the inva-sion, migration, nutrition and the survival of parasite and are key targets for novel drugs and vaccines. the aim of study was to determine the pro...

background: as a drug target and an antigenic agent, hiv-1 protease (hiv-1 pr) is at the center of attention for designing anti-aids inhibitors and diagnostic tests. in previous studies, the production of the recombinant protease has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of protease in e. coli and inves...

Proteolytic cleavage of von Willebrand factor (vWF) takes place in the circulating blood of healthy subjects and is increased in some patients with von Willebrand disease type 2A. The hemostatically active large vWF multimers are degraded to smaller less active forms. It has been suggested that the polypeptide subunit of vWF is cleaved at the peptide bond 842Tyr-843Met. We purified (approximate...