Inhibition of metabolism by structural analogs of metabolites is one of the newer concepts of chemotherapy. It seems possible that this concept might be applied to cancer by use of structural analogs of glucose to inhibit the glycolysis of the tumor cell, since tumor tissue in contrast to most normal tissues possesses the ability to glycolyze glucose at a high rate both anaerobically and aerobi...

PURPOSE Evaluation of tolerance, toxicity, and feasibility of combining large fraction (5 Gy) radiotherapy with 2-deoxy-D-glucose (2DG), an inhibitor of glucose transport and glycolysis, which has been shown to differentially inhibit repair of radiation damage in cancer cells. METHODS AND MATERIALS Twenty patients with supratentorial glioma (Grade 3/4), following surgery were treated with fou...

Cancer cells have long been known to exhibit metabolic reprogramming which involves a shift towards glycolysis. The enhanced glycolysis of cancer cells not only impose nutrient restriction in the tumor microenvironment, but also contribute to the acidic environment which is hostile to the invading anti-tumor immune cells. Hence, targeting the glycolysis dependency of tumor cells has been exploi...

Clear native electrophoresis and blue native electrophoresis are microscale techniques for the isolation of membrane protein complexes. The Coomassie Blue G-250 dye, used in blue native electrophoresis, interferes with in-gel fluorescence detection and in-gel catalytic activity assays. This problem can be overcome by omitting the dye in clear native electrophoresis. However, clear native electr...

Let X be a random variable which for simplicity we shall assume to have discrete values x and which has a probability distribution depending in a known way on an unknown real parameter A, (1) p (xIX) =Pr [X = xIA =X], A-itself being a random variable with a priori distribution function (2) G (X) =Pr [A-< X. The unconditional probability distribution of X is then given by (3) PG(x) =Pr[X=xI =fP(...

Personalized medicine is the most promising approaches in the treatment of various diseases, especially cancer. The use of appropriate biomarkers for personalized treatment has advantage over conventional therapeutics approach, as it confer maximum effectiveness with minimum side effect. Personalized treatment can be achieved by implementation of omic studies in clinical practices. Application ...

UNLABELLED Aerobic glycolysis that supports high proliferation rate and survival of tumor cells in unfavorable conditions is among fundamental features of tumor metabolism. The search for active modulators of energetic metabolism capable of suppressing tumor growth and metastasis could result in higher effectiveness of anticancer therapy. AIM To study antitumor and antimetastatic activity of ...

The recent upsurge in proteomics research has been facilitated largely by streamlining of two-dimensional (2-D) gel technology and the parallel development of facile mass spectrometry for analysis of peptides and proteins. However, application of these technologies to the mitochondrial proteome has been limited due to the considerable complement of hydrophobic membrane proteins in mitochondria,...

Every human being is different based on genetics, lifestyle, and environmental factors. Novel&nbsp;medical technologies have become more precise owing to molecular information, including genomics, transcriptomics, proteomics, metabolomics, etc. The &ldquo;omics&rdquo; technologies have opened up new horizons for healthcare systems, enabling them to prevent and/or diagnose diseases more precisel...

Proteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge to use a tissue for proteomics studies is the small sample size and difficulties to extract and preserve proteins. The choice of a buffer compatible with proteomics applications is also a challenge. Here we describe a protocol optimized for the most efficient extraction of prot...