The spawn of cultivated mushrooms are generally produced, propagated, and distributed to growers as a mycelial culture without genetic purification, in which phenotypic variants frequently occur. We investigated how heterologous mycelia present in a spawn influence fruit body production in the cultivated basidiomycete Pholiota nameko. The 'di-mon' dual cultivation of protoplast clones produced ...

The genetic engineering of plants allows for the modification of plant genomes, subsequently improving plant traits. There are, however, plants recalcitrant to established transformation methods, requiring the implementation of novel techniques. This study investigates the viability of cell-penetrating peptide (CPP) transformation using Tat2, a CPP with demonstrated transformation efficiency in...

The freeze-fracture morphology of the plasma membrane of cells and isolated protoplasts of plant callus suspensions has been investigated. Plasmolysis of suspension cells leads to the formation of 2 types of hexagonal arrays of intramembrane particles situated on the inner fracture face (PF). These arrays are interpreted as proteins that have 'crystallized' in the plane of the membrane as the a...

Protoplast preparations made from Saccharomyces cerevisiae by prolonged treatment with snail digestive juice contained fibrils and chitinous bud-scar residues from the original cell wall.

Abstract Transient expression systems in mesophyll protoplasts have been utilised many plant species as an indispensable tool for gene function analysis and efficacious genome editing constructs. However, such a system has not developed Cannabis due to the recalcitrant nature of tissue culture well its illegal status years. In this study, young expanding leaves from aseptic vitro explants were ...