Wild plant species related to crop plants form an important source of useful traits for fruit quality improvement and biotic abiotic stress tolerance. Wide hybridization involving wild relatives taxa has gained momentum in the recent programs. It breaks barrier gene transfer makes it possible genome one another, which results changes genotypes phenotypes progenies. The incompatibilities or barr...

The use of in vitro potato shoot cultures for protoplast isolation is desirable for consistent protoplast qualities. Ethylene build-up in the culture vessels causes problems for leaf growth (e.g. decreasing leaf surface, dry weight and chlorophyll) in shoot culture. Fifty to one hundred µM Silver thiosulfate (STS) produced a larger leaf area as a source of plant material for protoplast isolatio...

The entomopathogenic fungal genus Lecanicillium Gams and Zare (formerly classified as the species Verticillium lecanii) includes that are highly pathogenic to many insect genera. In this study, we identified six Lecanicilliumspp. isolated strains (designated V1-V6) belonging L. lecanii (V1, V3 V5) attenuatum (V2, V4 V6). addition, these were used obtain new via protoplast fusion, nit mutants fo...

Wild Solanum species have often been used as sources of important agricultural traits, including resistance to various diseases, pests, and abiotic factors. However, their large-scale use in potato breeding is limited by complex barriers sexual incompatibility with tuberosum. Fusion protoplasts enzymatically isolated from somatic cells one the approaches overcoming incompatibility. The diverse ...

During the treatment of some plant tissues with cell-wall-degrading enzymes adjacent cells within the tissue fuse forming large multinucleate protoplasts. These have been termed spontaneous fusion bodies. The symplastic nature of plant tissues suggests that the retention of plasmodesmatal connexions might facilitate such spontaneous fusion. An electron-microscopic investigation of spontaneous f...