BACKGROUND Colorectal cancer (CRC) is a leading cause of death in the western world, and its incidence increases with patient age. It is also known that with age there occur changes in the levels of certain hormones, including an increase in the secretion of pituitary gonadotropins (PtGs) as a result of the loss of gonadal hormone feedback. We recently reported that functional PtG receptors are...

Angiotensin II (ANG II) type 1 (AT(1)) receptors, similar to other G protein-coupled receptors, undergo desensitization and internalization, and potentially nuclear localization, subsequent to agonist interaction. Evidence suggests that the carboxy-terminal tail may be involved in receptor nuclear localization. In the present study, we examined the carboxy-terminal tail of the receptor for spec...

Abstract The difference between the skeletal muscle growth rates of Western and domestic breeds is remarkable, but potential regulatory mechanism involved still unclear. Numerous studies have pointed out that long intergenic noncoding RNA (lincRNA) plays a key role in development. This study used published Yorkshire (LW) Tibetan pig (TP) transcriptome data to explore possible lincRNA developmen...

Transcriptional gene silencing (TGS) is the mechanism generally thought by which heterochromatin effects silencing. However, recent discovery in fission yeast of a cis-acting posttranscriptional gene-silencing (cis-PTGS) pathway operated by the RNAi machinery at heterochromatin challenges the role of TGS in heterochromatic silencing. Here, we describe a multienzyme effector complex (termed SHRE...

Background: Thyroid surgery is associated with a number of surgical complications including recurrent laryngeal nerve (RLN) injury and hypoparathyroidism. The existing methods share the same principle—the mobilization thyroid from lateral side. aim this study was to evaluate safety novel technique thyroidectomy—tension-free thyroidectomy (TFT) based on medial approach nerves parathyroid glands ...

The past decade has seen intense scientific interest in non-coding RNAs. In particular, the discovery and subsequent exploitation of gene silencing via RNA interference (RNAi) has revolutionized the way in which gene expression is now studied and understood. It is now well established that post-transcriptional gene silencing (PTGS) by the microRNA (miRNA) and other RNAi-associated pathways repr...

In recent decades, availability of ample information of various plant genomes has reached the hand of plant researchers through genome sequencing and expressed sequence tag (EST) analysis that eventually facilitate the functional study of wide range of genes. The previous work on plant functional genomics were exclusively based on forward genetics; that is, identification of a mutant and subseq...

Double-stranded RNAs can suppress expression of homologous genes through an evolutionarily conserved process named RNA interference (RNAi) or post-transcriptional gene silencing (PTGS). One mechanism underlying silencing is degradation of target mRNAs by an RNP complex, which contains approximately 22 nt of siRNAs as guides to substrate selection. A bidentate nuclease called Dicer has been impl...