Background: Evaluation of cell viability is momentous in pharmacologic and oncological research. Cell viability evaluation determines cell sensitivity and consequently treatment outcome. Various methods are available to determine cell survival. Each of these methods evaluates different endpoints. Accordingly, determining the correlation between these methods is important. In this study, in orde...

A nonlinear vibration system, over a span of convincing periodic motion, might break out abruptly catastrophic instability, but the lack theoretical tool has obscured prediction outbreak. This paper deploys amplitude-frequency formulation for oscillators to reveal critically important mechanism pseudo-periodic and finds quadratic force contributes pull-down phenomenon in each cycle when reaches...

OBJECTIVE The aim of this study was to assess sensitivity and specificity of surrogate physical ability tests as predictors of criterion firefighting task performance and to identify corresponding minimum muscular strength and endurance standards. METHODS Fifty-one (26 male; 25 female) participants completed three criterion tasks (ladder lift, ladder lower, ladder extension) and three corresp...

OBJECTIVE The objective of the present investigation was to investigate the role of integrin-linked kinase (ILK) in the gemcitabine-resistant lung cancer cell line A549 and explore the underlying mechanism. MATERIALS AND METHODS Gemcitabine-resistant A549 (A549/GemR) cell line was established by pulse-exposed to moderate concentration of gemcitabine (Gem), and the drug resistant index was mea...

PURPOSE Tissue factor (TF) plays an important role in neovascularization (NV). This study aimed to determine whether small interfering RNA-targeting TF (TF-siRNA) could knock down TF expression and inhibit cell proliferation, cell migration, and tube formation in an in vitro model of NV. METHODS Lipopolysaccharide (LPS) was used to stimulate human umbilical vein endothelial cell (HUVEC) lines...

OBJECTIVES Histone deacetylases (HDACs) plays important roles in the regulation of genes expression and contribute to the growth of cancer cells. The present study aimed to investigate the function of HDAC5 in human hepatocellular carcinoma (HCC). PATIENTS AND METHODS The expression of HDAC5 in human hepatocellular carcinoma tissues and cells was detected. MTT assay was used to measure the pr...

1. Abstract 2. Introduction 3. Materials and methods 3.1. Main reagents and cell culture 3.2. MTT assay 3.3. Comet assay 3.4. Cell cycle analysis by flow cytometry 3.5. The Caspase-3 colorimetric activity assay 3.6. Statistical analysis 4. Results 4.1. MTT assay 4.2. Comet assay 4.3. FCM assay the distribution of cell cycle and cell apoptosis 4.4. The expression of Caspase-3 5. Discussion 6. Ac...

background: various mtt assay methods are proposed to obtain the cell sur- vival parameters. objective: determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel mtt assay method after exposing them to ionizing radiation. method: a common and a novel mtt assay method were used and compared for obtaining the f10b16 melanoma and 4t1 breast ade...