BACKGROUND Outpatient treatment of uncomplicated acute diverticulitis is safe and effective. The aim of this study was to determine the impact of outpatient treatment on the reduction of healthcare costs. PATIENTS AND METHODS A retrospective cohort study comparing 2 groups was performed. In the outpatient treatment group, patients diagnosed with uncomplicated acute diverticulitis were treated...

Joanne Beck, PhD, director of biologics manufacturing, Abbott Research Center Uwe Gottschalk, PhD, vice president, purification technology, Sartorius AG, Germany David W. Kahn, PhD, director, late-stage purification development, Human Genome Sciences, Inc. Duncan Low, PhD, scientific director, process development, Amgen Inc. Jill A Meyers, PhD, principal consultant, BioPro Consulting Charles Sc...

This review focusses on affinity purification of immunoglobulins, a methodology which is a powerful tool to obtain pure and intact antibodies. Affinity techniques allow antibody purification both in a single step chromatographic procedure as well as in complex purification protocols depending on the intention to use the target antibody. The purification strategies for antibodies by interaction ...

The biocompatibilities in blood purification therapy are defined as "a concept to stipulate safety of blood purification therapy by an index based on interaction in the body arising from blood purification therapy itself." The biocompatibilities are associated with not only materials to be used but also many factors such as sterilization method and eluted substance. It is often evaluated based ...

Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...

The standard Akta Explorer high-performance liquid chromatography (HPLC) system has limitations for the automation of multidimensional protein purification. Here, we describe simple modifications that allow for automated multidimensional purification protocols to extend the possibilities of the Akta three-dimensional purification kit in terms of column number, flexibility of volumes stocked for...

Bromelain is one of the vegetal proteases found in pineapple plant. It has numerous applications in food and pharmaceuticals. This review discussed different bromelain purification techniques which will assist in determining the effect of processing conditions on the purification efficacy. There are four purification techniques to be discussed, namely; reverse micellar system, aqueous two phase...

The generation of clinical good manufacturing practices (GMP)-grade adeno-associated virus (AAV) vectors requires purification strategies that support the generation of vectors of high purity, and that exhibit a good safety and efficacy profile. To date, most reported purification schemas are serotype dependent, requiring method development for each AAV gene therapy product. Here, we describe a...

Reducing carbon dioxide to value-added chemicals by electrolysis is a promising strategy substitute fossil-based processes. Research on CO2 has vastly progressed, focusing catalysis and electrode design, leaving an essential question the central part of electrolyzer: Which type ion exchange membrane best suited for from economic perspective? To address this question, holistic process optimizati...

Membrane processes, whose low energy and operating costs make them competitive for gas separation compared to other conventional methods such as pressure swing adsorption, cryogenic distillation or amine absorption, allows high-purity be obtained in a sustainable continuous manner. Similarly, resin-derived carbon membranes with molecular sieving properties have improved the selectivity permeabi...