Materials-L-Threonine, L-serine, and pyridosal-P monohydrate were purchased from the California Corporation for Biochemical Research, a-ketobutyric acid from the Nutritional Biochemical Corporation, pyruvic acid from the Fisher Scientific Company, and DESE-cellulose from Eastman Organic Chemicals. nn-Allothreonine and L-allothreonine were kindly furnished by Dr. Alton iLleister of Tufts Univers...

In 1937, Sir H. A Krebs first published the Citric Acid Cycle, a unidirectional cycle with carboxylic acids. The original concept of Cycle from Krebs’ 1953 Nobel Prize lecture illustrates degradation lactic acid to water, carbon dioxide and hydrogen. Here, we add heart lactate dehydrogenase•proton-linked monocarboxylate transporter 1 complex, connecting flow energy material. complex catalyses r...

Bayesian truth serum (BTS) is an exciting new method for improving honesty and information quality in multiple-choice survey, but, despite the method's mathematical reliance on large sample sizes, existing literature about BTS only focuses on small experiments. Combined with the prevalence of online survey platforms, such as Amazon's Mechanical Turk, which facilitate surveys with hundreds or th...

Reaction 1 occurring with reaction 2 involves carbon dioxide fixation. Koepsell & Johnson (1942) obtained a cell-free extract from Clo8tridium butylicum which converts pyruvic acid into acetic acid, carbon dioxide and hydrogen, and requires phosphate for the reaction. Later Koepsell, Johnson & Meek (1944) found that inorganic phosphate is taken up and labile phosphate appears. The overall react...

A sensitive and specific assay for hyaluronate was devised. Hyaluronate contained in biological mixtures was digested with a commercially available microbial hyaluronate lyase. The P-A4,5-eneglucopyranuronic acid residues contained at the nonreducing termini of the resulting oligosaccharides were oxidized with periodic acid to yield, among other products, formyl pyruvic acid. The latter compoun...

Bacterial metabolism of Tyr and Phe has been associated with the formation of aromatic compounds that impart barny-utensil and floral off-flavors in cheese. In an effort to identify possible mechanisms for the origin of these compounds in Cheddar cheese, we investigated Tyr and Phe catabolism by Lactobacillus casei and Lactobacillus helveticus cheese flavor adjuncts under simulated Cheddar chee...

O-polysaccharides were isolated from the lipopolysaccharides of Escherichia coli O40 and Shigella dysenteriae type 9 and studied by chemical analyses along with (1)H and (13)C NMR spectroscopy. The following new structure of the O-polysaccharide of E. coli O40 was established: -->2)-beta-D-Galp-(1-->4)-beta-D-Manp-(1-->4)-alpha-D-Galp-(1-->3)-beta-D-GlcpNAc-(1--> TheO-polysaccharide structure o...

Determination of pyruvic acid and of glucose in the blood was done by Friedemann's (1) modification of the Lu method (2) and the Benedict method (3), respectively. The control group consisted of 28 healthy men ranging in age from 25 to 50 years. The series investigated by means of intravenously administered glucose included 15 patients with portal cirrhosis of the liver (three in hepatic coma),...

FOLLOWING the establishment [Platt & Lu, 1939] of the occurrence of increased amounts of pyruvic acid in the body fluids of patients suffering from beri-beri and the studies of Lu [1939] on the effect of muscular activity on the blood pyruvate of the rabbit, it appeared desirable that an investigation should be made of the relationships between pyruvic acid accumulation in the blood, amount of ...

The synthesis, structural characterization and the successful application of a carbon centered radical derived from 1,3-bisdiphenylene-2-phenylallyl (BDPA), its benzyl alcohol derivative (BA-BDPA), as a polarizing agent for Dynamic Nuclear Polarization (DNP) are described. The reported BA-BDPA radical meets all the requirements to become a promising candidate for its use in in vivo DNP-NMR expe...