BACKGROUND We previously reported that the one-step nucleic acid amplification assay is effective for lymph node metastasis detection in breast cancer patients. This paper describes the identification of CK19 mRNA as an optimal marker and its cut-off value for use in the detection of one-step nucleic acid amplification-based lymph node metastasis in colorectal cancer patients. METHODS Candida...

PURPOSE The main goal of sentinel lymph node (SLN) detection in head and neck squamous cell carcinomas is to limit neck dissections to pN+ cases only. However, intraoperative + diagnosis cannot be routinely done using the current gold standard, serial step sectioning with immunohistochemistry. Real-time quantitative reverse transcription-PCR (RT-PCR) is potentially compatible with intraoperativ...

UNLABELLED Our aim was to ascertain the relationship between the degree of 99mTc-MIBI uptake and the level of p-glycoprotein (Pgp) expression determined by flow cytometry and reverse transcription-polymerase chain reaction (RT-PCR) techniques in patients with hematologic malignancy. METHODS A total of 21 samples (19 patients) were evaluated. Two patients had repeat studies after therapy. Thir...

Koblitz curves have been a nice subject of consideration for both theoretical and practical interests. The window τ -adic algorithm of Solinas (window τNAF) is the most powerful method for computing point multiplication for Koblitz curves. Precomputation plays an important role in improving the performance of point multiplication. In this paper, the concept of optimal pre-computation for window...