The present study was conducted to investigate the effects of butylated hydroxytoluene (BHT) supplementation on diluted, cooled and frozen-thawed ram spermatozoa. After primary evaluation of collected ejaculates, only semen samples with motility of more than 70% and sperm concentration higher than 3×10 sperm/ml were used for cryopreservation. The selected semen samples were then pooled and dilu...

The primary cause of sperm quality decline during the freeze-thaw pathway is peroxidation hazard caused by reactive oxygen species produced biological molecules sperm. Ascorbic acid (Vitamin C) and lycopene are two potent antioxidants that operate to prevent oxidation processes. This study aimed analyse effects ascorbic on motility, viability, abnormality plasma membrane integrity post-thawed S...

Objective: This study was conducted to evaluate the pathological effects of experimental trypanosomosis on the semen collection reaction time and spermatozoa morphology of Yankasa rams. Materials and Methods: Twelve apparently healthy Yankasa rams aging 24-30 months and weighing 22-25 Kg were randomly selected and were distributed into four (4) groups. Groups I and II were challenged with exper...

The occurrence of membrane vesicles along the male reproductive tract and in the ejaculated semen appears as a common feature among different species, including humans. Indeed, prostasomes (prostate-derived vesicles) were first described in human semen in 1978 (Ronquist et al, 1978), and vesicular structures similar to prostasomes were also found in the seminal plasma of rabbit (Davis, 1978), r...

BACKGROUND Mammalian semen contains a family of closely related proteins known as Binder of SPerm (BSP proteins) that are added to sperm at ejaculation. BSP proteins extract lipids from the sperm membrane thereby extensively modifying its composition. These changes can ultimately be detrimental to sperm storage. We have demonstrated that bovine BSP proteins interact with major milk proteins and...

The study evaluated the effect of two animal protein-free extenders on ram semen cryopreservation tropical hair breeds. ejaculates were collected from 8 rams (4 Blackbelly and 4 Pelibuey), which mixed (pooled) by breed, diluted cryopreserved in three different extenders: Animal protein [Tris egg yolk (Triladyl®)], AndroMed® (lecithin soy bean) OPTIXcell® (liposome). Sperm analyses total (TM) pr...

This study investigated the effect of spirulina ethanolic extract (SEE) on quality ram semen during low-temperature liquid storage and relationship between sperm features. Ejaculates were collected from five Djallonké rams, pooled, extended with Tris-egg yolk (TEY) enriched 0 (control), 20 (SEE20), 40 (SEE40), 80 µg/mL (SEE80) SEE to reach concentration 200×106 spz/mL, stored at 4 °C for 72 h. ...