The aim of the present study was to develop a treatment supporting the membrane of ram spermatozoa. Semen of different ejaculates collected from breeding rams was mixed and samples of 10(9) sperm cells per ml and Tris-egg yolk extender were completed with the following antioxidants: alpha-tocopherol acetate (E), glutathione peroxidase (GP), Aromex (AR), resveratrol (R), resveratrol + vitamin E ...

The aim of this study was to evaluate the effects of the addition of antioxidants (vitamin E, β- mercaptoethanol and their combination) to semen extender.  Progressive motility, viability and pH of fresh ram semen were examined. Ejaculate samples were collected with artificial vagina from three Ghezel-Merinose rams (3 years old) during non- breeding reproduction season. Semen was diluted with a...

The present study investigated the possibility of replacing salamon with modified shotor diluent (MSD) and egg yolk (EY) with low density lipoprotein (LDL) for chilled storage of ram semen. Good quality semen (>80% progressive forward motility (PFM) of sperm) from 3 fertile rams was collected using an artificial vagina and pooled for each experiment. Low density lipoprotein was extracted from f...

objective: adiponectin and its receptors (adipor1 and adipor2), known as adiponectin system, have some proven roles in the fat and glucose metabolisms. several studies have shown that adiponectin can be considered as a candidate in linking metabolism to testicular function. in this regard, we evaluated the correlation between sperm mrna abundance of adiponectin and its receptors, with sperm mot...

Insulin is present in the seminal plasma and involved sperm activities like motility capacitation. However, effects of insulin on viability cooled ram are not fully understood. Therefore, objective current study was to evaluate effect addition maintained at 5ºC. Sperm samples were collected from six healthy, mature Santa Inês rams. The ejaculates divided into two aliquots with (insulin group) o...

Peroxisome proliferator-activated receptors (PPARs) are a member of nuclear receptors superfamily, which mainly regulate the expression of target genes involved in lipid and energy metabolism. These receptors are divided to three isotypes: PPARα, PPARγ and PPARβ/δ. Each isotype has a distinct tissue distribution relating to the distinct functions. In this study, the mRNA abundance for PPARα, PP...

Incubation of hamster eggs with trypsin or chymotrypsin, but not with a series of glycosidases or lipases, prevents capacitated spermatozoa from binding to the zona pellucida so that fertilization is prevented (Hartmann & Gwatkin, 1971 ; Gwatkin, Williams & Andersen, 1973). Inactivation of the binding sites for spermatozoa on the zona pellucida by a trypsin-like protease released by discharge o...

the aim of this study was to evaluate the effects of the addition of antioxidants (vitamin e, β- mercaptoethanol and their combination) to semen extender.  progressive motility, viability and ph of fresh ram semen were examined. ejaculate samples were collected with artificial vagina from three ghezel-merinose rams (3 years old) during non- breeding reproduction season. semen was diluted with a...

The aim of this study was to evaluate the effects of the addition of antioxidants (vitamin E, β- mercaptoethanol and their combination) to semen extender.  Progressive motility, viability and pH of fresh ram semen were examined. Ejaculate samples were collected with artificial vagina from three Ghezel-Merinose rams (3 years old) during non- breeding reproduction season. Semen was diluted with a...