brucellosis is one of the most common zoonotic diseases in iran and human brucellosis is endemic in all parts of the country. growth of brucella is slow and blood culture of these bacteria by use of classical methods is time-consuming. furthermore, in endemic area culture is required for definitive diagnosis. in the present study, direct urease test and acridine orange staining were tried on th...

The influence of urease activity on N distribution and losses after foliar urea application was investigated using wild-type and transgenic potato (Solanum tuberosum cv Désirée) plants in which urease activity was down-regulated. A good correlation between urease activity and (15)N urea metabolism (NH(3) accumulation) was found. The general accumulation of ammonium in leaves treated with urea i...

BACKGROUND Pathogen-specific metabolic pathways may be detected by breath tests based on introduction of stable isotopically-labeled substrates and detection of labeled products in exhaled breath using portable infrared spectrometers. METHODOLOGY/PRINCIPAL FINDINGS We tested whether mycobacterial urease activity could be utilized in such a breath test format as the basis of a novel biomarker ...

Detection and intensity of urease activity in enterobacteriaceae greatly varies as a function of the media or techniques used, or both. A comparative investigation on several solid and liquid media led us to the following conclusions. (i) Detection of Proteus spp. can be adequately performed with the highly selective solid medium described by Cook (1948), as well as with the different liquid me...

Baicalin (BA) is the principal component of Radix Scutellariae responsible for its pharmacological activity. In this study, kinetics and mechanism of inhibition by BA against jack-bean urease were investigated for its therapeutic potential. It was revealed that the IC₅₀ of BA against jack-bean urease was 2.74 ± 0.51 mM, which was proved to be a competitive and concentration-dependent inhibition...

BACKGROUND The rapid urease test (RUT) is used at Groote Schuur Hospital for diagnosing Helicobacter pylori infection. This is an in-house method, which has not been validated. OBJECTIVE To validate our practice of reading the RUT immediately after endoscopy (RUT(0)), by comparing this with a reading at 24 hours (RUT(24)) and with histological analysis. DESIGN Ninety consecutive patients un...

The influence of urease activity on N distribution and losses after foliar urea application was investigated using wild-type and transgenic potato (Solanum tuberosum cv Désirée) plants in which urease activity was down-regulated. A good correlation between urease activity and N urea metabolism (NH3 accumulation) was found. The general accumulation of ammonium in leaves treated with urea indicat...

BACKGROUND The purpose of this study was to determine the sensitivity, specificity, and positive and negative predictive values of a newly developed paper urease test (PUT) for ultra-rapid detection of helicobacter pylori (HP) in gastric mucosa. MATERIAL/METHODS 100 patients (43 men) with a mean age of 57+/-6.8 years participated. Patients presenting for upper endoscopy with no recent exposur...

infection is found in more than 50% of humans, and the infection rate among adults in South Korea exceeds 60%. 1 For that reason, guidelines have been established for the management of H. pylori including the diagnostic method, determining who should be treated, and the recommended treatment. 2-4 In regions with low H. pylori prevalence , a test-and-treat strategy is usually recommend for those...