A new spray reagent with copper, ammonium molybdate, and sulphuric acid for the detection of phospholipids on thin-layer chromatograms is described. The specificity and sensitivity of the spray reagent is also shown.

A novel proton acceptor coupling reagent shows superiority to those described previously. The oxygen in the carbocation moiety confers more solubility to the reagent. Furthermore, it enhances coupling yields and decreases racemization, allowing the use of 1 equiv of base.

In connection with the study of the state of the calcium of the blood, we desired a rapid method for determining serum proteins. Wu (1) and Wu and Ling (2) have published a method for the determination of plasma proteins based on the color developed with Folin’s phenol reagent. The method as described by these authors is not very satisfactory due to the formation of turbid precipitates and the ...

The use of protein reagents which exhibit sensitivity to changes in molecular environment has provided a means of examining certain properties of enzymes during interaction with their substrates (1). The recently reported 2-hydroxy-5nitrobenzyl bromide provided one such reagent, the absorption spectrum of which showed sensitivity to environmental changes by virtue of the p-nitrophenolic groupin...

BACKGROUND The favorable properties of lanthanide chelates compared with conventional fluorescent probes have attracted considerable interest. A Eu(3+) chelator, 4,7-bis(chlorosulfophenyl)-1, 10-phenanthroline-2,9-dicarboxylic acid (BCPDA), has been synthesized previously. METHODS We here describe immunoassay, immunohistochemistry, and microarray applications of a new streptavidin-based unive...

Our objective was to investigate the protective effect of Lawesson's reagent, an H2S donor, against alendronate (ALD)-induced gastric damage in rats. Rats were pretreated with saline or Lawesson's reagent (3, 9, or 27 µmol/kg, po) once daily for 4 days. After 30 min, gastric damage was induced by ALD (30 mg/kg) administration by gavage. On the last day of treatment, the animals were killed 4 h ...

BACKGROUND The improved reagent for measuring estradiol (E2), the ST AIA-PACK iE2 reagent, has a higher specificity for the measurement of E2 levels than the original ST AIA-PACK E2 reagent, because of its lower cross-reactivity with estrone (E1). As we had E2 data obtained with either of the reagents, we analyzed changes in E1 and E2 levels during follicle development. METHODS The study incl...

Local calibration studies have been performed with lyophilized plasmas at 155 European laboratories to assess coagulometer effects on manual ISI of ECAA thromboplastins (low ISI human recombinant and high ISI rabbit brain). Common sets of 7 normal and 20 artificially depleted lyophilized plasmas were tested with the thromboplastins in the routine local coagulometers. With the human reagent, mar...

aspect to take into consideration when choosing among reagents. Reconstituting a fresh vial of reagent daily dramatically increases costs when the majority of a previous vial is discarded. All reagents tested were relatively inexpensive, costing an average of US $3.00/mL (list price). In summary, all assays detected hypofibrinogenemia, although the BioMerieux reagent yielded slightly prolonged ...