Initiator tRNAs are used exclusively for initiation of protein synthesis and not for elongation. We show that both Escherichia coli and eukaryotic initiator tRNAs have negative determinants, at the same positions, that block their activity in elongation. The primary negative determinant in E. coli initiator tRNA is the C1xA72 mismatch at the end of the acceptor stem. The primary negative determ...

Background Redox regulation system protects our body from oxidative stress-injury and keeps redox homeostasis. The hydrogen molecules (H2) exist as stable gas in the ordinal temperature and atmosphere. Recent study reports H2 improve ischemia-reperfusion injury, glaucoma, Parkinson’s disease and atherosclerosis of animal models. It is supposed from these improvement results that H2 participate ...

A new protocol for carcinogenesis in rat liver is described in order that confirmatory experiments might be undertaken concurrently. The basic protocol, designated IPI (initiator + promoter + initiator), is presented in several alternative forms, including the possible use of X-irradiation as the initiator. The rationale is discussed in terms of the two-hit somatic mutation theory of Armitage a...

Commercial initiator – 2,2-dimethoxy-2-phenyl acetophenone (Irgacure 651, Ciba, Switzerland) was introduced to poly(methyl methacrylate) (PMMA) matrix to enhance the polymer degradation. Two types of thin polymeric films (PMMA and PMMA+5% Irgacure) were UV-irradiated in laboratory conditions (Xenon lamp, air atmosphere, room temperature). The photochemical changes in pure PMMA and PMMA doped wi...

In prokaryotes, many of the physical mechanisms governing the process of initiating DNA replication are now emerging. For example, certain organizational features of origins, such as the use of repetitive sequence elements for initiator-binding sites, are found throughout bacteria and many archaea. Common themes in the regulation of initiation, including origin sequestration by trans-acting fac...

Transcription associated with a terminal deoxynucleotide transferase gene initiator element is shown to respond to the transcription factor GAL4-VP16 both in vivo and in vitro. High-level transcription requires both an intact initiator element and bound activator. Transcription from this initiator-directed promoter is synergistic in vivo in that five GAL4 DNA binding sites yield 36 times the ex...

styrene polymerization through frp and atrp methods was carried out at 110oc, while in-depth studies were performed by monte carlo simulation.the changes in monomer conversion, initiator concentration, average molecular weight, and polydispersity index were computed over the course of the polymerization. as the results indicate, compared to atrp, the frp reaches higher conversion in a similar r...

The characteristics and kinetics of redox transformation of a redox mediator, anthraquinone-2-sulfonate (AQS), during microbial goethite reduction by Shewanella decolorationis S12, a dissimilatory iron reduction bacterium (DIRB), were investigated to provide insights into "redox mediator-iron oxide" interaction in the presence of DIRB. Two pre-incubation reaction systems of the "strain S12- goe...

We report the redox properties of four star systems incorporating the 4-benzoyl-N-alkylpyridinium cation; the redox potential varies along the branches but remains constant at fixed radii. Bulk electrolysis shows that at a semi-infinite time scale all redox centers are electrochemically accessible. However, voltammetric analysis (cyclic voltammetry and differential pulse voltammetry) shows that...