Calcium-dependent release of purines was previously demonstrated in cultures of chick retinal cells stimulated with high potassium concentrations but there is no evidence for an exocytotic mechanism of adenosine release from presynaptic terminals. Here we show that activation of NMDA or alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA)/kainate glutamate ionotropic receptors promotes a...

We studied how Ca2+ influx through different subtypes of Ca2+ channels couples to release at a calyx-type terminal in the rat medial nucleus of the trapezoid body by simultaneously measuring the presynaptic Ca2+ influx evoked by a single action potential and the EPSC. Application of subtype-specific toxins showed that Ca2+ channels of the P/Q-, N-, and R-type controlled glutamate release at a s...

BACKGROUND The greater nitric oxide (NO) release that occurs in the internal mammary artery (IMA) when compared with the saphenous vein (SV) has been suggested by more endothelium-dependent relaxation in the IMA or measured by bioassay; however, no direct measurement of NO- or endothelium-derived hyperpolarizing factor (EDHF)-mediated hyperpolarization has been reported. The present study measu...

Purpose: To determine whether Myofascial Release (MFR) technique in upper cervical region is more effective than using conventional exercises to improve cervical muscle strength in patients with cervicogenic headache. Design: Randomized Controlled Trial. Setting: An outpatient physical therapy clinic, University of social welfare and rehabilitation science, Iran. Methods: We conducted a prospe...

j J Am Med Inform Assoc. 2006;13:30–39. DOI 10.1197/jamia.M1888. Clinical Document Architecture, Release One (CDA R1), became an American National Standards Institute (ANSI)– approved Health Level 7 (HL7) Standard in November 2000, representing the first specification derived from the HL7 Reference Information Model (RIM). CDA, Release Two (CDA R2), became an ANSI-approved HL7 standard in May 2...

ackground: Sex differences in addictive disorders have been described. Preclinical studies have implicated the striatal dopamine ystem in these differences, but human studies have yet to substantiate these findings. ethods: Using positron emission tomography (PET) scans with high-specific-activity [C] raclopride and a reference tissue approach, e compared baseline striatal dopamine binding pote...

To examine the role of the macula densa in renin release, afferent arterioles alone or afferent arterioles with the macula densa attached were microdissected from rabbit kidney and incubated in Medium 199 for two consecutive 30-minute periods. Renin concentration in the medium was measured using partially purified rabbit angiotensinogen. Renin release rate over 1 hour from a single arteriole (o...

We evaluated the possible role of NO in modulating tissue plasminogen activator (t-PA) release in the forearm microcirculation of normotensive subjects and hypertensive patients. Essential hypertensive patients are characterized by endothelial dysfunction because of a reduced NO availability and also show an impaired t-PA release. In healthy volunteers and essential hypertensive patients, we st...

The release of the opioid antagonist naltrexone from neutral poly(N-isopropylacrylamide) (PNIPAAM) microgels and negatively charged PNIPAAM microgels containing acrylic acid groups (PNIPAAM-co-PAA) has been studied at various microgel and drug concentrations. The release curves were found to be well represented by the Weibull equation. The release rates were observed to be dependent on the micr...

Oligomerization of all three mammalian ryanodine receptor isoforms, a structural requirement for normal intracellular Ca2+ release channel function, is displayed by the discrete N-terminal domain which assembles into homo- and hetero-tetramers. This is demonstrated in yeast, mammalian cells and native tissue by complementary yeast two-hybrid, chemical cross-linking and co-immunoprecipitation as...