Determination of sex origin of cattle meat by fast and reliable molecular methods is an important measure to ensure correct allocation of export refunds particularly in European countries and also female cattle (cow) slaughter is legally banned in India because of religious beliefs. Based on the DEAD box protein gene located on the X and Y chromosomes, 2 pair of primers were designed and the sy...

Listeria monocytogenes is an important foodborne pathogen. The aims of this study were to determine genetic relatedness of L. monocytogenes isolated from ready-to-eat (RTE) foods in Malaysia. L. monocytogenes isolates from RTE foods were characterized by multiplex-PCR serotyping, REP-PCR, BOX-PCR, RAPD, PFGE, virulotyping and antibiotyping. Of the 32 L. monocytogenes isolates analyzed, 21 (65.6...

in this paper, full scale load test of a horizontally curved steel box-girder bridge is carried out in order to detect structural defects, which reportedly result in unwanted vibrations in nearby buildings. the bridge is tested under the passage of six heavy vehicles at different speeds, so as to determine its static and dynamic responses. a total number of one hundred and two (102) sensors are...

BACKGROUND Enteric Redmouth Disease (ERM), caused by Yersinia ruckeri, is one of the most important infectious diseases in rainbow trout (Oncorhynchus mykiss) aquaculture in Europe. More recently, non-motile vaccine resistant isolates appear to have evolved and are causing disease problems throughout Europe, including Germany. The aim of this study was to analyse the variation of biochemical an...

REP 2139 is a nucleic acid polymer (NAP) currently under clinical development for chronic hepatitis B (HBV) therapy. This preclinical study investigated different REP 2139 analogs that would display reduced accumulation in the serum and tissues, while retaining an antiviral effect against HBV infection. REP 2139 analogs were evaluated in human plasma, CD-1 mice, cynomolgus monkeys, and Pekin du...

The putative replication initiation protein (Rep) of Coconut foliar decay virus (CFDV) was expressed as a 6x His recombinant protein in E. coli and in recombinant baculovirus. Purified 6x His-Rep protein was demonstrated to possess sequence non-specific RNA- and ssDNA-binding activities as well as magnesium-dependent ATPase/GTPase activity. The yeast two-hybrid system revealed that CFDV Rep cou...