Optical Mapping is an established single-molecule, whole-genome analysis system, which has been used to gain a comprehensive understanding of genomic structure and to study structural variation of complex genomes. A critical component of Optical Mapping system is the image processing module, which extracts single molecule restriction maps from image datasets of immobilized, restriction digested...

The random-breakage mapping method [Game et al. (1990) Nucleic Acids Res., 18, 4453-4461] was applied to DNA sequences in human fibroblasts. The methodology involves NotI restriction endonuclease digestion of DNA from irradiated calls, followed by pulsed-field gel electrophoresis, Southern blotting and hybridization with DNA probes recognizing the single copy sequences of interest. The Southern...

Recently, low and high resolution restriction maps of the entire E. coli chromosome were constructed (1,2). This allows rapid physical mapping of genes on the chromosome. Efficient use of the high resolution restriction map awaits the availability of computerized version of the genomic map and a method for aligning restriction map data on it. Here, we describe a simple and efficient method for ...