transforming growth factor betas are multifunctional polypeptides in the cytokine superfamily. they have a growth inhibitory role on hemopoietic progenitor cells in semisolid colony assay as well as in long-term bone-marrow culture. tgf - β2 represses stromal cells, stem cell factor gene transcription, and decreases the stability of c-kit transcripts in hemopoietic cells. tgf-β also modulates g...

We consider the problem of building cost-effective networks which are robust to dynamic changes in demand patterns. We compare several architectures based on using demandoblivious routing strategies. Traditional approaches include single-hop architectures based on a (static or dynamic) circuitswitched core infrastructure, and multi-hop (packet-switched) architectures based on point-to-point cir...

Red leaf blotch (RLB) of almond, caused by Polystigma amygdalinum, is an important foliar disease this nut tree in the Mediterranean basin and especially Spain. In recent years, control has become a key factor management Spanish almond orchards. The RLB not easy due to intrinsic factors (e.g., long infection latency periods) low number registered fungicides country. From 2015 2019, different fi...

Background and Aims: Viroids are smallest known plant pathogens and cause several economically significant diseases. Until recently, viroid detection relied mainly on biological tests and indexing. Today various diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (RT-PCR) are being used for detection and diag...

Little is known about the occurrence of haemoparasites in cattle in communal grazing areas of Mungwi District of Northern Province, Zambia. Clinical signs and post mortem lesions are pathognomonic of mixed tick-borne infections especially babesiosis, anaplasmosis and East Coast fever. The main objective of this study was to screen selected communal herds of cattle for tick-borne haemoparasites,...

The paper aimed to investigate the effects of up-regulation and down-regulation Mir-299-3p on proliferation, migration, invasion apoptosis lung adenocarcinoma cell line H1299, preliminarily explore mechanism regulating biological behavior cells. inhibitor mimics were transfected into transfection efficiency was measured by fluorescence microscopy. successfully cells detected invasion, apoptosis...

Based upon reverse transcription and polymerase chain reaction results with human KB cell RNA, a cDNA (i.e., 3'rTS1, 1557 nt) with complementarity to thymidylate synthase mRNA was cloned and sequenced. Northern blot analysis showed that 3'rTS1 corresponded to a cytoplasmic 1.8 kb RNA found in several tumor cell lines. The remaining 5'region of this antisense RNA was cloned by a RACE (Rapid Ampl...

PURPOSE The mechanism of resistance to cisplatin during treatment of bladder cancer (BC) has been a subject of intense investigation in clinical research. This study aims to identify candidate genes associated with resistance to cisplatin, in order to understand the resistance mechanism of BC cells to the drug, by combining the use of microarray profiling, quantitative reverse transcription-pol...

Purpose: To investigate the mechanism of microRNA-124 action on neuroblastoma apoptosis induced by sevoflurane.
 Methods: MiR-124 expression was assessed in a cell line (SMS-KAN) using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The role miR-124 sevoflurane anesthesia-induced studied activity and analysis 3-(4, 5-dimethylthiazolyl-2-yl)-2-5 diphenyl tetrazolium ...

We investigated the expression of peroxisome proliferator-activated receptor gamma (PPARgamma) and the role of PPARgamma in cell growth in human gastric cancer cells. Reverse transcription-polymerase chain reaction, Northern blot and Western blot analyses showed that a human gastric cancer cell line, MKN45, expressed PPARgamma mRNA and protein. Luciferase assay in MKN45 cells showed that trogli...