The experiment on “Effect of fungicides Sclerotium rolfsii isolates groundnut from Khandesh region” was conducted at Plant Pathology Section, College Agriculture, Dhule. results obtained are presented here under. Groundnut (Arachis hypogea L.) is a major edible oil seed crop tropical and subtropical region the world. It world’s fourth important source third vegetable protein also seeds, vines d...

Rhizobium is a bacterial culture that enhances the speed of biological nitrogen fixation in association with root nodules pulse crops while boron act as catalyst for cell division hence size and sugar translocation plant. An experiment was conducted by considering rhizobium seed inoculant soil applicant two peas (Pisum sativum L.) varieties Arkel Azad Pea-3 to know morphological alterations pla...

We have developed a simple system to clone indigenous Rhizobium plasmids into E. coli. The strategy consists of three matings: the first is to insert Tn5 in the plasmid to be cloned, the second incorporates the integrative vector into the inserted Tn5 in the native Rhizobium plasmid, and the last mating transfers the target plasmid directly into E. coli. This mating-based system was successfull...

Mutualisms are known to be genetically variable, where the genotypes differ in the fitness benefits they gain from the interaction. To date, little is known about the loci that underlie such genetic variation in fitness or whether the loci influencing fitness are partner specific, and depend on the genotype of the interaction partner. In the legume-rhizobium mutualism, one set of potential cand...

Symbiotically essential genes have been identified in Rhizobium meliloti that are structurally and functionally related to chromosomal virulence (chv) genes of Agrobacterium tumefaciens. Homologous sequences also exist in the genomes of other fast-growing rhizobia including Rhizobium trifolii, Rhizobium leguminosarum, and Rhizobium phaseoli. In Agrobacterium, the chvA and chvB loci are known to...

Lateral transfer of bacterial plasmids is thought to play an important role in microbial evolution and population dynamics. However, this assumption is based primarily on investigations of medically or agriculturally important bacterial species. To explore the role of lateral transfer in the evolution of bacterial systems not under intensive, human-mediated selection, we examined the associatio...

Crown gall, caused by Agrobacterium tumefaciens (syn. Rhizobium radiobacter), is characterized gall formation on crowns, stems or roots many plant species. We describe the occurrence of this disease for first time cannabis (Cannabis sativa L. marijuana) plants in two licenced production facilities British Columbia. Galls were observed and several genotypes, including ‘White Rhino’, ‘Pink Kush’ ...