نتایج جستجو برای: rhizobium leguminosarum
تعداد نتایج: 5242 فیلتر نتایج به سال:
Mutant strains of Rhizobium trifolii that induce nitrogenase activity in the free-living state were isolated. These strains, unlike the wild type, nodulated soybean and mungbean plants, producing effective nodules. One of the strains, strain DT72, also nodulated clover but the nodules were ineffective. Strain DT125 consumed hydrogen gas from the gas phase, which can be coupled to nitrogenase ac...
We isolated RNA polymerase (RNAP) from Rhizobium leguminosarum, the nitrogen-fixing symbiont of peas and VICIA: Its 91 kDa subunit, which is homologous to sigma(70) of Escherichia coli RNAP, is necessary for transcription of the regulatory nodD gene, which in the presence of certain flavonoids induces transcription of other nod genes that are needed for the early steps of infection. We also sho...
Identification of a NodD repressible gene adjacent to nodM in Rhizobium leguminosarum biovar viciae.
The nodFEL and nodMNT operons in Rhizobium leguminosarum biovar viciae are transcribed in the same orientation and induced by NodD in response to flavonoids secreted by legumes. In the narrow intergenic region between nodFEL and nodMNT, we identified a small gene divergently transcribed from nodM to the 3' end of nodL. Unlike the promoters upstream of nodF and nodM, the promoter of this gene is...
Rhizobium leguminosarum bv trifolii is the effective nitrogen fixing microsymbiont of a diverse range of annual and perennial Trifolium (clover) species. Strain WSM2304 is an aerobic, motile, non-spore forming, Gram-negative rod, isolated from Trifolium polymorphum in Uruguay in 1998. This microsymbiont predominated in the perennial grasslands of Glencoe Research Station, in Uruguay, to competi...
The symbiotic characteristics of Rhizobium trifolii strains 1-01 and 2-01 were evaluated both individually and in various combinations on two cultivars (Mt. Barker and Woogenellup) of subterranean clover (Trifolium subterraneum L.). Nodules were observed on day 8 independent of cultivar or strain. Cultivar differences were measured in nodulating efficiency by 1-01 since 54% of the primary nodul...
Symbiotically essential genes have been identified in Rhizobium meliloti that are structurally and functionally related to chromosomal virulence (chv) genes of Agrobacterium tumefaciens. Homologous sequences also exist in the genomes of other fast-growing rhizobia including Rhizobium trifolii, Rhizobium leguminosarum, and Rhizobium phaseoli. In Agrobacterium, the chvA and chvB loci are known to...
The phylogenetic relatedness among 12 agriculturally important species in the order Rhizobiales was estimated by comparative 16S rRNA and dnaK sequence analyses. Two groups of related species were identified by neighbor-joining and maximum-parsimony analysis. One group consisted of Mesorhizobium loti and Mesorhizobium ciceri, and the other group consisted of Agrobacterium rhizogenes, Rhizobium ...
Symbiotic DNA sequences involved in nodulation by Rhizobium must include genes responsible for recognizing homologous hosts. We sought these genes by mobilizing the symbiotic plasmid of a broad host-range Rhizobium MPIK3030 (= NGR234) that can nodulate Glycine max, Psophocarpus tetragonolobus, Vigna unguiculata, etc., into two Nod- Rhizobium mutants as well as into Agrobacterium tumefaciens. Su...
Rhizobium leguminosarum has two high-affinity Mn2+ transport systems encoded by sitABCD and mntH. In symbiosis, sitABCD and mntH were expressed throughout nodules and also strongly induced in Mn2+ -limited cultures of free-living cells. Growth of a sitA mntH double mutant was severely reduced under Mn2+ limitation and sitA and mntH single mutants were more sensitive to oxidative stress. The dou...
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