The extracellular acid phosphatase (EC 3.1.3.2) of Rhodotomla glutinis is a glycoenzyme composed of 608 protein, 35% mannose, and 5% N-acetylglucosamine by weight. The native enzyme elutes from a column of BioGel A-5M agarose with an M, 5 185,000 but on sodium dodecyl sulfate-acrylamide gel electrophoresis migrates with an M, = 93,000. The acid phosphatase thus appears to consist of two subun...
