A fragment of the 16S RNA of Thermus thermophilus corresponding to the central domain (nucleotides 547-895) has been prepared by transcription in vitro. Incubation of this fragment with the total 30S ribosomal proteins has resulted in the formation of a compact 12S ribonucleoprotein particle. This particle contained five T. thermophilus proteins corresponding to Escherichia coli ribosomal prote...

Introduction This Auxiliary Material contains detailed discussions of our study sites and of each coral head analyzed. We methodically describe the Bunon sites (Text S1 and Figures S1–S12) and Pulau Penyu site (Text S2 and Figures S13–S18), and we explain the construction of relative sea-level histories, considering multiple scenarios where data are ambiguous. Supplementary tables provide detai...

Department of Chemistry, University of Zu Zurich, Switzerland. E-mail: gilles.ga gassergroup.com; Tel: +41 44 635 46 30 Helmholtz-Zentrum Dresden – Rossendorf, Research, Bautzner Landstraße 400, [email protected]; Web: http://www.hzdr.d 3091 Lehrstuhl für Anorganische Chemie I – Bioa und Biochemie, Ruhr-Universität Bochum, U Germany † Electronic supplementary information PNAs used this study (Fi...

The Bacillus subtilis genome comprises two paralogous single-stranded DNA binding protein (SSB) genes, ssb and ywpH, which show distinct expression patterns. The main ssb gene is strongly expressed during exponential growth and is coregulated with genes encoding the ribosomal proteins S6 and S18. The gene organization rpsF-ssb-rpsR as observed in B. subtilis is found in many gram-positive as we...

Abstract To realize the integrated remediation of SW/GW and soil in rural river network area, area project (IR-RRNA), funded by Ministry Science Technology People’s Republic China, has been launched. In eastern (RRNA) is an anthropic active characterized its rapid economic development high gross national product. However, water environmental pollution these areas increasingly severe, which grea...

Specific hybridization assays for intermediates in rRNA synthesis (pre-rRNA) may become useful for monitoring the growth activity of individual microbial species in complex natural systems. This possibility depends upon the assumption that rRNA processing in microbial cells continues after growth and pre-rRNA synthesis cease, resulting in drainage of the pre-rRNA pool. This is not the case in m...

BACKGROUND Ribosomal RNA (rRNA) is a central regulator of cell growth and may control cancer development. A cis noncoding rRNA (nc-rRNA) upstream from the 45S rRNA transcription start site has recently been implicated in control of rRNA transcription in mouse fibroblasts. We investigated whether a similar nc-rRNA might be expressed in human cancer epithelial cells, and related to any genomic ch...