A new coronavirus (SARS-CoV-2) caused the current disease (Covid-19) epidemic. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is used as gold standard for clinical detection of SARS-CoV-2. Under ideal conditions, RT-qPCR Covid-19 assays have analytical sensitivity and specificity greater than 95%. However, when sample panel enlarged including asymptomatic individuals, de...

Biomarker changes between primary (PT) and metastatic tumor (MT) site may be significant in individualizing treatment strategies and can result from actual clonal evolution, biomarker conversion, or technical limitations of diagnostic tests.This study explored biomarker conversion during breast cancer (BC) progression in 67 patients with different tumor subtypes and metastatic sites via mRNA qu...

An analytical system based on rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR) for enumeration of catalase-negative, Gram-positive cocci was established. Subgroup- or species-specific primer sets targeting 16S or 23S rRNA from Enterococcus, Streptococcus, and Lactococcus were newly developed. The RT-qPCR method using these primers together with the previously reported primer sets ...

Human noroviruses are a major cause for gastroenteritis outbreaks. Filter-feeding bivalve molluscs, which accumulate in their digestive tissues, typical vector human infection. RT-qPCR, the established method norovirus detection food, does not allow discrimination between infectious and non-infectious viruses can overestimate potentially viral loads. To develop more accurate of load estimation,...

Abstract A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and being used for screening procedure all incoming passengers to Northern Cyprus SARS-CoV-2. This study investigated compatibility of two different RT-qPCR methodologies Diagnovital® Bio-Speedy® by re-analyzing previously confirmed positive samples. total 43 samp...

The SARS-CoV-2 pandemic has forced all countries worldwide to rapidly develop and implement widespread testing control manage the Coronavirus Disease 2019 (COVID-19). reverse-transcription (RT)-qPCR is gold standard molecular diagnostic method for COVID-19, mostly in automated platforms. These systems are accurate effective, but also costly, time-consuming, high-technological, infrastructure-de...

Real-time quantitative PCR (RT-qPCR) has been widely applied in uncovering disease mechanisms and screening potential biomarkers. Internal reference gene selection determines the accuracy reproducibility of data analyses. The aim this study was to identify optimal genes for relative analysis RT-qPCR fourteen NF1 related cell lines, including non-tumor, benign malignant Schwann lines. expression...

Wastewater-based surveillance methods have been implemented in several countries as a tool for monitoring SARS-CoV-2 at community scale. A variety of used concentrating, extracting, and detecting the virus, with no clear consensus on most effective approach. In this note, we report preliminary findings from study that is tracking wastewater Halifax, Nova Scotia, specific focus use four reverse ...