OBJECTIVE This study was designed to evaluate the effects of vitrification and in vitro culture of human ovarian tissue on the expression of oocytic and follicular cell-related genes. MATERIALS AND METHODS In this experimental study, ovarian tissue samples were obtained from eight transsexual women. Samples were cut into small fragments and were then assigned to vitrified and non-vitrified gr...

Six strain of Trichoderma (T. viride, T. harzianum, T. reesei, T. longibrachiatum, T. koningii, T. virens) were isolated and used for extracellular enzyme production. Species of Trichoderma are collected from the surrounding soil of suger beet root fields in East Iran, and held in vitro cultured PDA. TCM and TFM are used for the growth of spores and induceing the production of Cellulase. Finall...

Basidiocarps of species of Armillaria, Russula, Lactarius and  Agaricus with fungal infection were collected from different regions of Iran. Portions of infected carpophores, were taken and cultured on PDA, PCA and MEA. Fungicolous isolates were identified on the basis of morphological characters such as: color and shape of colony, morphology and branching of conidiophores, manner of proliferat...

Rapid and effective methods for the isolation of Clostridium difficile from stool samples are desirable to obtain isolates for typing or to facilitate accurate diagnosis of C. difficile-associated diarrhea. We report on the evaluation of a prototype chromogenic medium (ID C. difficile prototype [IDCd]) for isolation of C. difficile. The chromogenic medium was compared using (i) 368 untreated st...

Introduction: Resistance of pathogenic organisms to countenance antibiotics has become a worldwide problem with serious consequences on the treatment of infectious diseases. The aim of this study was to evaluate antibiotic resistance and also the detection of transferred antibiotic resistance by plasmid in clinical Proteus isolates. Materials and methods: A total of 250 urine samples were ...

conclusions a relatively high prevalence of acanthamoeba spp. in rivers reflects a risk alert for threatening human health in the region. however, well hygienic status of the tap waters considering acanthamoeba spp. cannot be ignored in western co-border regions of iran-iraq. this study can also serve as a platform for further explorations of water sources in iran and neighboring countries. res...

in vitro multiple shoot regeneration of solanum nigrum l., an indian medicinal plant was accomplished on ms medium utilizing shoot tip and nodal explants. direct multiple shoots differentiated within 6 weeks when explants were cultured on ms medium containing bap (1.0-5.0 mg/l) and kin (1.0-5.0 mg/l) individually. among various concentrations of cytokinins tested, maximum number of multiple sho...

objective: listeria monocytogenes is a facultative, gram-positive bacterium which is found in soil, water, decaying vegetables, raw milk, and contaminated dairies. listeria monocytogenes causes listeriosis. listeriosis is a zoonosis disease, which transfers from animals to humans by animal feces and contaminated dairies. listeriosis causes the flu like disease or self-limited enteritis, but it ...