Alstroemeria pelegrina (A. pelegrina), a Chilean endemic, is considered vulnerable as its natural habitat currently threatened. The decline in the reproductive capacity of species due to anthropogenic impacts and climate change has made it imperative address problem by developing large-scale propagation methods. objective this study was establish protocols for breaking dormancy vitro germinatio...

Tissue slices of zucchini squash (Cucurbita pepo L., cv. Tigress) fruit were exposed to ultraviolet-C (UV-C) radiation from germicidal lamps for 1, 10 or 20 min; however, only 10 and 20 min UV-C exposure significantly reduced microbial activity and deterioration during subsequent storage at 5 or 10 degrees C. UV-C treated slices had higher respiration rates than controls; however, the ethylene ...

PURPOSE To evaluate the antiviral activity of 2', 3'-dideoxycytidine (ddC) in vitro against a panel of ocular adenovirus serotypes and in vivo in the ocular Ad5/NZW rabbit replication model. METHODS In vitro, the 50% inhibitory concentrations (IC(50)) of ddC and cidofovir were determined using standard plaque-reduction assays. In vivo, 40 rabbits were topically inoculated in both eyes with Ad...

Cultivation of chilling-tolerant ornamental crops at lower temperature could reduce the energy demands of heated greenhouses. To provide a better understanding of how sub-optimal temperatures (12°C vs. 16°C) affect growth of the sensitive Petunia hybrida cultivar 'SweetSunshine Williams', the transcriptome, carbohydrate metabolism, and phytohormone homeostasis were monitored in aerial plant par...

A combination of hot water (a rinse at 62 degrees C for 20 s) and conditioning (pre-storage at 16 degrees C for 7 d) treatments synergistically reduced chilling injury development in grapefruit (Citrus paradisi, cv. "Star Ruby") during cold storage at 2 degrees C, suggesting that the treatments may activate different chilling tolerance responses. To study the molecular mechanisms involved, chil...

Infectious bovine keratoconjunctivitis (IBK) is a common ocular disease in cattle, associated with a 6.8 to 13.6 kg decrease in weaning weight. Antibiotic therapy is available but it is unclear if pain mitigation as an adjunct therapy would reduce the weight loss associated with IBK. Before assessing the impact of pain mitigation therapies, it is first necessary to validate approaches to qualif...

The regulatory mechanisms underlying bud breaking (scale leaf elongation) and flowering in the lateral flower buds of Japanese pear (Pyrus pyrifolia Nakai 'Kosui') are unknown. To more fully characterize these processes, we treated pear trees with different amounts of chilling initiated at different times. Chilling for ∼900 h at 6 °C always induced bud breaking (scale elongation in ≥70% lateral...

Spores of foodborne pathogens can survive traditional thermal processing schedules used in the manufacturing of processed meat products. Heat-activated spores can germinate and grow to hazardous levels when these products are improperly chilled. Germination and outgrowth of Clostridium perfringens spores in roast beef during chilling was studied following simulated cooling schedules normally us...

It was assumed that one of the most important factors preventing the hibernation of the diamondback moth, Plutella xylostella (L.), in northern Japan is the low temperatures, 0 ° C or below, and snow cover during the winter. To test this hypothesis, individuals of each developmental stage of diamondback moth were reared at 1 5 ° C in the laboratory and kept at 0°C for varying periods of time an...