The present study was designed to see whether adding glutathione to tris-citric egg yolk extender improves the quality of buffalo bull spermatozoa stored at 5°C for five days. Semen collected from Nili-Ravi buffalo (Bubalus bubalis) bulls (n = 5) was diluted (37C; 10 x 10 motile spermatozoa per ml) with extender having glutathione (0.0, 0.5, 1.0, 2.0 and 3.0mM). The extended semen was cooled fr...

the present study was aimed to test the efficacy of adding vitamins c or e to tris-fructose-egg yolk diluent to increase awassi ram sperm storage period at 5 ˚c. semen samples from six mature awassi rams were used in this study. the semen samples were diluted by tris-glucose-egg yolk. diluted semen sample was divided into three parts. the first part was added with 0.9 mg ml-1 vitamin c, the sec...

Introduction Although dog was the first species to be used for Artificial Insemination (AI) and become pregnant by Spallanzani in 1780, very little work has been done on preservation of dog semen to exploit the full genetic potential of pedigree males. Frozen semen is now inseminated routinely in cattle, sheep and goats and much research has been carried out with regard to the optimum condition...

The present study was aimed to test the efficacy of adding vitamins C or E to Tris-fructose-egg yolk diluent to increase Awassi ram sperm storage period at 5 ˚C. Semen samples from six mature Awassi rams were used in this study. The semen samples were diluted by Tris-glucose-egg yolk. Diluted semen sample was divided into three parts. The first part was added with 0.9 mg mL(-1) vitamin C, the s...

This study was carried out to determine if a new antibiotic combination comprising of gentamycin, tylosin and linco-spectin (GTLS) in extender is suitable for improvement in fertility of liquid buffalo bull semen through artificial insemination (AI). Two consecutive ejaculates per week (4 weeks) were collected from three Nili-Ravi buffalo bulls of known fertility by using artificial vagina (42°...

The comet assay, under neutral conditions, allows the assessment of DNA integrity influenced by sperm ageing, which is manifested in DNA double-strand breaks. Here, we attempted to use a modified neutral comet assay test (single-cell gel electrophoresis), to our knowledge for the first time, to assess DNA integrity of boar spermatozoa during liquid storage for 96 h at 5 ̊C and 16 ̊C. In this come...

Goats are small ruminants that reared by the rural community for financial income and nutrition. This study was aimed to determine effect of vitamin C addition in tris–egg yolk extender on lifespan Sapera goat spermatozoa stored 5o C. Semen collected from 1.5 years old male goat. The ejaculates were diluted Tris-egg (T-EY) added with 0, 0.2, 0.3, 0.4 g C/mL T0, T1, T2, T3 groups, respectively. ...

Caffeine is a well-known sperm motility stimulator, however, its effects on cooling-stored ram semen are unknown. The aim of the study was to examine the effect of caffeine on selected motility and viability indices of cooling-stored ram spermatozoa. Sperm ejaculates from 4 rams were diluted (1:3) in a Triladyl extender. Samples were stored for 96 h at 4-5 °C in two sets. In the first set used ...