The separation of neutral and water-insoluble linear synthetic polymers in non-aqueous capillary zone electrophoresis using the hydrophobic phosphonium-based ionic liquid trihexyl(tetradecyl)phosphonium chloride (P66614Cl) were investigated using polystyrene, polymethylmethacrylates, polybutadiene, and polycarbonate as test analytes. The solubility of P66614Cl in tetrahydrofuran was above 500 m...

The scope of this work was to investigate the migration behavior of the currently used protease inhibitors for antiretroviral therapy of people infected with the human immunodeficiency virus and to develop a method for their capillary electrophoretic separation and determination. All of the protease inhibitors (indinavir, saquinavir, nelfinavir, amprenavir, and ritonavir) contain at least one b...

By THOMAS G. FERRIS, ROBERT E. EASTERLING AND RICHARD E. BUDD T HE VARIOUS types of hemoglobins have been identified and reported by means of zone electrophoresis using supporting media such as filter paper,’ starch,2 agar,3 cellulose acetate,4 and combinations of these media. Recently a synthetic acrylamide gel ( Cyanogum 41#{176} ) has been investigated and found to have characteristics which...

Synthesis and characterization of a polymethine cyanine near-infrared (NIR) fluorophoric label for the derivatization and determination of fatty acids separated by capillary electrophoresis are described. The label contains an aromatic amine functionality, which is used to form a covalent linkage with the analyte. Various linking chemistries are explored, including direct amine–acid condensatio...

Electrophoresis of a solute through a column in which its transport is governed by the convection diffusion equation is described. Approximate solutions to the convection difision equation in the limit of small diffusion are developed using perturbation methods. The difision coeficient and velocity are assumed to be functions of space and time such that both undergo a sudden change from one con...

Monosaccharides are transported across the human erythrocyte membrane by a facilitated diffusion mechanism mediated by intrinsic membrane protein. The identification of the protein(s) involved has been approached from specific sugar binding experiments and reconstitution studies in liposomes and planar bilayer membranes (Jones & Nickson, 1981) but there have been considerable difficulties in un...

The growth of compositionally uniform alloy crystals is promising for variety of applications because lattice parameters as well as electrical and optical properties can be controlled by composition. Among them, InxGa1-xAs bulk crystals are expected as substrates of laser diodes with emitting wavelength of 1.3 μm. High optical gain with small temperature dependence was demonstrated for strained...

Articular and growth plate cartilage are discrete tissues but arise from a common cartilaginous condensation and have comparable spatial architectures consisting of distinct layers of chondrocytes. To investigate similarities and differences between articular and growth plate cartilage and to explore transcriptional changes that occur during the onset of their divergence, we performed manual mi...