Rho family GTPases such as Cdc42 are key regulators of essential cellular processes through their effects on cytoskeletal dynamics, signaling and gene expression. Rho GTPases modulate these functions by engaging a wide variety of downstream effectors. Among these effectors is the largely understudied Cdc42EP/BORG family of Cdc42 effectors. BORG proteins have been linked to actin and septin regu...

The eukaryotic cytosolic chaperonin containing TCP-1 (CCT) has an important function in maintaining cellular homoeostasis by assisting the folding of many proteins, including the cytoskeletal components actin and tubulin. Yet the nature of the proteins and cellular pathways dependent on CCT function has not been established globally. Here, we use proteomic and genomic approaches to define CCT i...

Members of the septin gene family are involved in cytokinesis and the organization of new growth in organisms as diverse as yeast, fruit fly, worm, mouse, and human. Five septin genes have been cloned and sequenced from the model filamentous fungus A. nidulans. As expected, the A. nidulans septins contain the highly conserved GTP binding and coiled-coil domains seen in other septins. On the bas...

Septin filaments assemble into high-order molecular structures that associate with membranes, acting as diffusion barriers and scaffold proteins crucial for many cellular processes. However, how septin organize in such is still not well understood. In this study, we used fission yeast to explore filament organization during cell division decipher key factors responsible their regulation. Live-i...

Septins are a cytosolic GTP-binding protein family first characterized in yeast, but gaining increasing recognition as critical protagonists in higher eukaryotic cellular events. Mammalian septins have been associated with cytokinesis and exocytosis, along with contributing to the development of neurological disorders. Ten different septins, divided into four groups, have been identified in mam...

Septins are a family of GTP-binding proteins that, in most fungi and animals, form hetero-octameric rods containing two copies each of four different family members. These rods can then assemble into higher-order structures that perform a variety of cellular functions, depending on their subunit composition. The human genome, for example, encodes 13 different septins expressed by specifi c cell...

ART 1102 Methodologies in the History and Theory of Art (3 units) Introduction to a variety of theoretical, critical, and historical methods used in art history and art criticism. Preparation in recognizing and utilizing particular methodologies in scholarly research, professional work, and writing. Examination of primary and secondary theoretical texts from a range of historical eras to judge ...

Septins are filamentous GTPases that polymerize from hetero-oligomeric complexes. Their order was based on crystallographic studies, but we provide evidence the canonical organization is inverted. This revised septin congruent with and behavior of yeast septins, suggesting their properties conserved.

Mitotic yeast (Saccharomyces cerevisiae) cells express five related septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1) that form a cortical filamentous collar at the mother-bud neck necessary for normal morphogenesis and cytokinesis. All five possess an N-terminal GTPase domain and, except for Cdc10, a C-terminal extension (CTE) containing a predicted coiled coil. Here, we show that the CTEs of Cdc3...