AIM To determine the involvement of apoptotic cell death in postnatal pathogenesis in mutant strain of hypogonadic (hgn/hgn) rats testes. We evaluated the numbers and types of cells undergoing apoptotic cell death. METHODS Tissue sections were stained by the TUNEL method for in situ detection of apoptotic cells, with specific antibodies used as markers of testicular somatic and germ cells. ...

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous and persistent environmental contaminants. Some PAHs are carcinogens and may affect the male reproductive system. Therefore, we exposed cultured rat Sertoli cells to a variety of PAHs to determine possible direct toxic effects on the cells of the seminiferous epithelium. Sertoli cells were chosen because they support germ cell development a...

Abstract Autoimmune diseases play a critical role in the progression of infertility both sexes and their severity has been reported to increase with age. However, few reports have discussed effect on morphological features testis. Therefore, we compared alterations testes autoimmune model mice (MRL/MpJ-Fas lpr ) control strain (MRL/MpJ) those background (C57BL/6N) at 3 6 months. Furthermore, an...

Sertoli cells, can function as non-professional tolerogenic antigen-presenting cells, and sustain the blood-testis barrier formed by their tight junctions. The NOD-like receptor family members and the NALP3 inflammasome play a key role in pro-inflammatory innate immunity signalling pathways. Limited data exist on NOD1 and NOD2 expression in human and mouse Sertoli cells. Currently, there is no ...

Androgen and androgen receptor (AR) play important roles in male spermatogenesis and fertility, yet detailed androgen/AR signals in Sertoli cells remain unclear. To identify AR target genes in Sertoli cells, we analyzed the gene expression profiles of testis between mice lacking AR in Sertoli cells (S-AR(-/y)) and their littermate wild-type (WT) mice. Digital gene expression analysis identified...

The met protooncogene encodes the hepatocyte growth factor receptor (HGFR, c-met). C-met, a tyrosine kinase receptor protein, is widely expressed in different cell types including the male reproductive tract. As we recently demonstrated, both c-met messenger RNA and protein are expressed in prebuberal rat testis. The aim of this work was to detect the expression of c-met during postnatal testis...

Experimental hypothyroidism during sexual development Testes size and number of spermatozoa in adulthood are determined by postnatal/pubertal proliferation of seminal tubule somatic cell constituents, the Sertoli cells. Cessation of proliferation of Sertoli cells during early development occurs concomitantly with formation of their terminally differentiated population. Transient experimental hy...

OBJECTIVES We sought to study the renoprotective effect of cotransplanted allogeneic testicular Sertoli cells on renal acute rejection in rats. MATERIALS AND METHODS A renal acute rejection model using kidneys from Sprague-Dawley (n=30) transplanted into Wistar rats (n=30) was constructed. The rats were randomly divided into 3 groups: (1) the cyclosporine group, which was treated with daily h...

The androgen testosterone is essential for the Sertoli cell to support the maturation of male germ cells and the production of spermatozoa (spermatogenesis). In the classical view of androgen action, binding of androgen to the intracellular androgen receptor (AR) produces a conformational change in AR such that the receptor-steroid complex has high affinity for specific DNA regulatory elements ...

1. About 19 per cent. of the dry weight of the normal pig testicle is fatty matter. Histologically this fat occurs largely in the cells of the seminal tubules, and especially in the Sertoli cells. 2. During spermatogenesis the fat of the Sertoli cell passes inward for the nutrition of the spermatids and spermatozoa. During this passage its character is altered from a neutral fat to a lipoid. 3....