Physalis is an herbaceous species native to the Andes region. Currently, it cultivated in various Brazilian states due economic interest of growers for this new fruit. plants grown field showed symptoms shoot proliferation, leaf malformation, and chlorosis. Since these are commonly induced by phytoplasmas, study investigated confirm presence prokaryotes symptomatic plants. After DNA extraction ...

fig trees are threatened by the attack of fig mosaic disease (fmd) on leaves and fruits caused by viruses of several genera. shoot-tip culture is a convenient method for viral sanitation. for this purpose, a reliable protocol for rapid in vitro propagation was developed with shoot-tips of three major tunisian local fig (ficus carica l.) varieties zidi (zdi), soltani (sni), bither abiadh (ba) an...

Achillea species are of high medicinal value based on their use in traditional medicine and content secondary metabolites with potential application dermatology cosmetic industry. occulta is a single-mountain local prioritized vulnerable Greek endemic encountering propagation problems through seeds root division. In vitro can be used for the rapid mass production clonal plants complying phytoch...

Plant root encounters coarse environs right after emergence from the seed. Little is known about metabolic changes enabling to overcome soil impedance. It also not whether roots respond differently hardness when grown in horizontal and vertical orientation. We grew tomato (Solanum lycopersicum) seedlings vertically or horizontally on agarose with increasing (0.2 % → 1.2 %). examined morphology ...

In vitro culture of Dioscorea wightii belongs to the family Dioscoreace. An efficient micropropagation protocol was developed for the medicinal plant Dioscorea wightii using nodal segments for axillary shoot proliferation. Callus initiation was stimulated at the basis of the explants in 100% of the cultures by 0.15-1.75μM BA, 0.75-5.0μM Kinetin, 0.15-0.30μM 2iP.The average fresh mass of calluse...

The shoot apical meristem is responsible for above-ground organ initiation in higher plants, accomplishing continuous organogenesis by maintaining a pool of undifferentiated cells and directing descendant cells toward organ formation. Normally, proliferation and differentiation are balanced, so that the structure and size of the shoot meristem is maintained. However, Arabidopsis plants homozygo...

In vitro plantlet regeneration has been obtained from 20-25days old seedling shoot tip segments of Cucumis melo (L.). We were examined using various phyto hormones individually and in combination on Murashige and Skoog (MS) semi solid medium supplemented with BAP (1.0-3.0 mg/L) Kn (1.0-3.0 mg/L),IAA (0.5 mg/L)+ BAP (1.0-3.0 mg/L) and IAA (0.5 mg/L)+Kn (1.0-3.0 mg/L) for multiple shoots prolifer...

Seeds of Bauhinia acuminata L. were used as an experimental material for micropropagation. The effect of growth regulators concentrations on morphogenetic development was studied. Seeds were germinated on MS medium supplemented with GA3 (0.10, 1.0 and 2.0 mgL -1 ) and without GA3. Satisfactory germination was observed at media with 1.0 mgL -l GA3. Subsequent propagation from plantlet was perfor...

Withania somnifera (L.) Dunal is an important ayurvedic medicinal plant known mainly for its rejuvenating properties. In vitro micropropagation or multiplication of Withania somnifera through apical shoot proliferation by using different concentrations of growth regulators in short period. Shoots were induced from the apical meristem of Withania somnifera on Murashige and Skoog (MS) basal media...

Arabidopsis amp1 mutants show pleiotropic phenotypes, including altered shoot apical meristems, increased cell proliferation, polycotyly, constitutive photomorphogenesis, early flowering time, increased levels of endogenous cytokinin, and increased cyclin cycD3 expression. We have isolated the AMP1 gene by map-based cloning. The AMP1 cDNA encodes a 706;-amino acid polypeptide with significant s...