نتایج جستجو برای: shotgun assembly sequences
تعداد نتایج: 336465 فیلتر نتایج به سال:
One consistent finding among studies using shotgun metagenomics to analyze whole viral communities is that most viral sequences show no significant homology to known sequences. Thus, bioinformatic analyses based on sequence collections such as GenBank nr, which are largely comprised of sequences from known organisms, tend to ignore a majority of sequences within most shotgun viral metagenome li...
Assembling fragments randomly sampled from along a sequence is the basis of whole-genome shotgun sequencing, a technique used to map the DNA of the human and other genomes. We calculate the probability that a random sequence can be recovered from a collection of overlapping fragments. We provide an exact solution for an infinite alphabet and in the case of constant overlaps. For the general pro...
Whole genome shotgun sequence analysis has become the standard method for beginning to determine a genome sequence. The preparation of the shotgun sequence clones is, in fact, a biological experiment. It determines which segments of the genome can be cloned into Escherichia coli and which cannot. By analyzing the complete set of sequences from such an experiment, it is possible to identify gene...
This paper presents a new mathematical programming model for the bi-criteria mixed-model assembly line balancing problem in a just-in-time (JIT) production system. There is a set of criteria to judge sequences of the product mix in terms of the effective utilization of the system. The primary goal of this model is to minimize the setup cost and the stoppage assembly line cost, simultaneously. B...
With the advent of capillary gel electrophoresis, DNA sequencing has become quite robust and reliable (1). Still, there has always been a need to sequence large segments of cloned DNA. Various methods have been devised over the years to accomplish such sequencing. The shotgun approach to DNA sequencing utilizes random fragmentation of cloned DNA that can then be subcloned into a bacteriophage M...
Mitochondrial genome (mitogenome) sequences are being generated with increasing speed due to the advances of next-generation sequencing (NGS) technology and associated analytical tools. However, detailed comparisons to explore the utility of alternative NGS approaches applied to the same taxa have not been undertaken. We compared a 'traditional' Sanger sequencing method with two NGS approaches ...
Group B streptococcus is known as a leading cause of neonatal infections in developing countries. The present study describes the whole genome shotgun sequences of four Group B Streptococcus (GBS) isolates. Molecular data on clonality is lacking for GBS in India. The present genome report will add important information on the scarce genome data of GBS and will help in deriving comparative genom...
Advances in sequencing technology have drastically increased the depth and feasibility of bacterial genome sequencing. However, little information is available that details the specific techniques and procedures employed during genome sequencing despite the large numbers of published genomes. Shotgun approaches employed by second-generation sequencing platforms has necessitated the development ...
This is the first of two lectures by Phil Green on “Sequence Assembly.” Yeast and some of the bacterial genomes have already been completely sequenced. There is also a rapidly growing body of known human sequence. All this has been because of our ability to read DNA. This lecture discusses the shotgun strategy for rapid DNA sequencing, and begins discussion of sequence assembly algorithms (to b...
A 2.91-billion base pair (bp) consensus sequence of the euchromatic portion of the human genome was generated by the whole-genome shotgun sequencing method. The 14.8-billion bp DNA sequence was generated over 9 months from 27,271,853 high-quality sequence reads (5.11-fold coverage of the genome) from both ends of plasmid clones made from the DNA of five individuals. Two assembly strategies-a wh...
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